The impact of increased consumption of fruits and vegetables on cortisol and cortisone excretion in 24-h urine of children
The impact of increased consumption of fruits and vegetables on cortisol and cortisone excretion in 24-h urine of children
复制标题
增加水果和蔬菜摄入量对儿童24小时尿液中皮质醇和可的松排泄的影响
DOI：
10.1055/s-0035-1547774
复制
发表时间：
2015
影响因子：
1.8
通讯作者：
Remer T
中科院分区：
文献类型：
--
作者：
Esche J;Sánchez-Guijo A;Hartmann MF;Wudy SA;Remer T
背景：通过施用矿物盐介导的年轻人碱化导致糖皮质激素（GC）代谢物排泄参数显着降低。然而，尚不清楚以增加水果和蔬菜 (f&v) 摄入量为特征并伴有平均蛋白质摄入量的碱性饮食是否会导致 GC 排泄减少。 方法：在 DONALD 研究的子样本 (n= 80) 中，对 6-10 岁的健康儿童进行了研究，并通过 3 天的饮食记录评估了他们的 f&v 摄入量。通过测量生物标志物总氮来确定蛋白质摄入量，并通过对 24 小时尿液中的尿游离皮质醇 (UFF) 和尿游离可的松 (UFE) 进行串联质谱分析来确定潜在生物活性的游离 GC。根据 f&v 摄入量，儿童被分为两组 (n= 40)，一组水果和蔬菜摄入量高 (f&v_h)，另一组水果和蔬菜摄入量低 (f&v_l)。为了确保蛋白质摄入量具有可比性，仅纳入那些 24 小时总氮排泄量为 380-540 mmol/m2/天的儿童。通过测量 24 小时肾净酸排泄 (NAE) 来评估每日酸负荷。通过 ANCOVA 分析两个 f&v 组之间 GC 排泄的差异（根据性别、体表面积、尿量和总氮进行调整）。结果：f&v 消耗量和 NAE（中位数：分别为 602 与 158 g/天和 22 与 41 mEq/天，P < 0.0001）在 f&v_h 和 f&v_l 内存在明显差异。此外，与 f&v_l 相比，f&v_h 显示出 ΣUFF+ UFE 和游离可的松 UFE 排泄量较低的趋势（ANCOVA；p≤ 0.1）。结论：这些初步初步结果表明，f&v 摄入量的增加可能与潜在生物活性游离 GC（ΣUFF+ UFE）的减少同时发生。基于更大的子样本，将测试潜在的混杂因素，然后将更详细地检查 f&v 消费与 GC 之间的关系。
Background: Alkalization of young adults mediated by an administration of mineral salt leads to a significant decrease of glucocorticoid (GC) metabolite excretion parameters. However, it is unclear, if already an alkaline diet, characterized by an increased fruit and vegetable (f&v) consumption, and accompanied by an average protein intake, causes a decrease of GC excretion.Methods: In a subsample (n= 80) of the DONALD study, 6–10 year-old healthy children were studied and their f&v consumption was assessed by a 3-day dietary record. Protein intake was determined by measurement of the biomarker total nitrogen and potentially bioactive free GCs were determined by tandem mass spectrometric analysis of urinary free cortisol (UFF) and urinary free cortisone (UFE) in 24-h urine. Based on the f&v consumption, the children were subdivided into two groups (n= 40), one with high (f&v_h) and one with low (f&v_l) fruit and vegetable intake. To ensure a comparable protein intake, only those children were included with a total 24-h nitrogen excretion of 380–540 mmol/m2/day. Daily acid load was assessed by measurement of 24-h renal net acid excretion (NAE). Differences of GC excretion between the two f&v groups were analysed by ANCOVA (adjusted for sex, body surface area, urinary volume, and total nitrogen).Results: f&v consumption and NAE (medians: 602 vs. 158 g/day and 22 vs. 41 mEq/day respectively, P< 0.0001) differed distinctly within f&v_h and f&v_l. Additionally, f&v_h showed a trend for a lower excretion of ΣUFF+ UFE and free cortisone UFE compared to f&v_l (ANCOVA; p≤ 0.1).Conclusions: These first preliminary results indicate that an increased intake of f&v may be paralleled by a decrease of potentially bioactive free GCs (ΣUFF+ UFE). Based on a larger subsample potential confounders will be tested and the–then–in more detail examined relationship between f&v consumption and GCs will be presented.