Chondroitinase injection improves keloid pathology by reorganizing the extracellular matrix with regenerated elastic fibers
Chondroitinase injection improves keloid pathology by reorganizing the extracellular matrix with regenerated elastic fibers
复制标题
DOI：
10.1111/1346-8138.12116
复制
发表时间：
2013-05-01
影响因子：
3.1
通讯作者：
Suzuki, Shigehiko
中科院分区：
文献类型：
--
作者：
Ishiko, Toshihiro;Naitoh, Motoko;Suzuki, Shigehiko
瘢痕疙瘩是一种增生性纤维性疾病，其特征是真皮中细胞外基质异常堆积。由于细胞外基质中弹性纤维的形成不足，瘢痕疙瘩皮损缺乏皮肤可塑性。弹性纤维的丧失是由于硫酸软骨素(CS)的过度积累造成的，CS是一种硫酸糖胺聚糖。然而，目前尚无根治瘢痕疙瘩的方法。利用一个模型系统，我们在这里展示了用软骨素酶ABC治疗瘢痕疙瘩组织，软骨素酶ABC是一种特异性地消化CS的酶，改善了瘢痕疙瘩的临床特征。将患者的瘢痕疙瘩组织移植到裸鼠身上，并在移植的瘢痕疙瘩组织中注射软骨素酶ABC。软骨素酶ABC治疗显著减少了瘢痕疙瘩植入物的体积，同时恢复了弹性纤维的形成。提示软骨素酶ABC注射液是治疗瘢痕疙瘩的有效方法。
Keloids are a proliferative fibrotic disease characterized by abnormal accumulation of extracellular matrix in the dermis. Keloid lesions lack skin plasticity due to deficiencies in elastic fiber formation in the extracellular matrix. The loss of elastic fiber is caused by excessive accumulation of chondroitin sulfate (CS), a sulfated glycosaminoglycan. However, there is no radical cure for keloids. Using a model system, we show herein that treatment of keloid tissues with chondroitinase ABC, an enzyme that specifically digests CS, improves clinical features of keloids. Keloid tissues obtained from patients were grafted on nude mice, and chondroitinase ABC was injected into the grafted keloid tissues. Chondroitinase ABC treatment significantly reduced the volume of keloid implants concomitant with recovery of elastic fiber formation. These results suggest that chondroitinase ABC injection is an effective therapy for keloid.