Protective Effects of Tormentic Acid, a Major Component of Suspension Cultures of Eriobotrya japonica Cells, on Acetaminophen-Induced Hepatotoxicity in Mice.
Protective Effects of Tormentic Acid, a Major Component of Suspension Cultures of Eriobotrya japonica Cells, on Acetaminophen-Induced Hepatotoxicity in Mice.
复制标题
DOI：
10.3390/molecules22050830
复制
发表时间：
2017-05-18
期刊：
影响因子：
--
通讯作者：
Huang GJ
中科院分区：
文献类型：
--
作者：
Jiang WP;Huang SS;Matsuda Y;Saito H;Uramaru N;Ho HY;Wu JB;Huang GJ
对乙酰氨基酚（APAP）过量可引起肝毒性并导致致命的肝损伤。研究了拷问酸（TA）对对乙酰氨基酚（APAP）所致小鼠肝损伤的保护作用。TA在APAP给药前腹腔注射6天。TA预处理可防止apap处理小鼠血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、总胆红素（T-Bil）、总胆固醇（TC）、甘油三酯（TG）和肝脂过氧化水平升高，并显著降低apap诱导的肝组织组织学改变。此外，TA还能减弱apap诱导的一氧化氮（NO）、活性氧（ROS）、肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)和IL-6的产生。Western blot分析显示，TA阻断了apap损伤肝组织中诱导型NO合成酶（iNOS）和环氧合酶-2 （COX-2）的蛋白表达，抑制了核因子κB （NF-κB）和丝裂原活化蛋白激酶（MAPKs）的活化。TA还保留了肝脏中的超氧化酶歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）。上述结果提示，TA的保肝作用可能与其降低硫代巴比妥酸反应物质（TBARS）、iNOS、COX-2、TNF-α、IL-1β、IL-6、抑制NF-κB和MAPK活化有关。肝脏中血红素氧化酶-1 （HO-1）的诱导，脂质过氧化物和ROS的减少也显示了抗氧化特性。因此，TA可能是通过抑制氧化应激和炎症来预防apap诱导的肝损伤的潜在治疗候选者。
An acetaminophen (APAP) overdose can cause hepatotoxicity and lead to fatal liver damage. The hepatoprotective effects of tormentic acid (TA) on acetaminophen (APAP)-induced liver damage were investigated in mice. TA was intraperitoneally (i.p.) administered for six days prior to APAP administration. Pretreatment with TA prevented the elevation of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (T-Bil), total cholesterol (TC), triacylglycerol (TG), and liver lipid peroxide levels in APAP-treated mice and markedly reduced APAP-induced histological alterations in liver tissues. Additionally, TA attenuated the APAP-induced production of nitric oxide (NO), reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and IL-6. Furthermore, the Western blot analysis showed that TA blocked the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as the inhibition of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) activation in APAP-injured liver tissues. TA also retained the superoxidase dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) in the liver. These results suggest that the hepatoprotective effects of TA may be related to its anti-inflammatory effect by decreasing thiobarbituric acid reactive substances (TBARS), iNOS, COX-2, TNF-α, IL-1β, and IL-6, and inhibiting NF-κB and MAPK activation. Antioxidative properties were also observed, as shown by heme oxygenase-1 (HO-1) induction in the liver, and decreases in lipid peroxides and ROS. Therefore, TA may be a potential therapeutic candidate for the prevention of APAP-induced liver injury by inhibiting oxidative stress and inflammation.
登录
查看更多内容
影响因子：
3.8
作者：
Du, Kuo;Williams, C. David;McGill, Mitchell R.;Jaeschke, Hartmut
通讯作者：
Jaeschke, Hartmut
影响因子：
4.1
作者：
Chen, Chi;Krausz, Kristopher W.;Shah, Yatrik M.;Idle, Jeffrey R.;Gonzalez, Frank J.
通讯作者：
Gonzalez, Frank J.
影响因子：
4.8
作者：
Carayol, Nathalie;Chen, Ji;Wang, Cun-Yu
通讯作者：
Wang, Cun-Yu
枇杷 (Eriobotrya japonica (Thunb.) Lindl.) 三萜酸的作用。
影响因子：
6.7
作者：
Huang, Y.;Li, J.;Wang, Y. -Y.
通讯作者：
Wang, Y. -Y.
中国传统健康酒朱耶克酒对小鼠急性酒精引起的肝损伤的保护作用。
DOI：
10.1186/1476-9255-10-30
发表时间：
2013-10-03
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
Gao HY;Huang J;Wang HY;Du XW;Cheng SM;Han Y;Wang LF;Li GY;Wang JH
通讯作者：
Wang JH