Feedback regulation of auxin signaling through the transcription of H2A.Z and deposition of H2A.Z to SMALL AUXIN UP RNAs in Arabidopsis.
Feedback regulation of auxin signaling through the transcription of H2A.Z and deposition of H2A.Z to SMALL AUXIN UP RNAs in Arabidopsis.
复制标题
在拟南芥中，通过 H2A.Z 的转录和 H2A.Z 沉积到小生长素 UP RNA 来反馈调节生长素信号。
DOI：
10.1111/nph.18440
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发表时间：
2022-08
期刊：
影响因子：
9.4
通讯作者：
Yuda Fang
中科院分区：
文献类型：
--
作者：
Aiqing Sun;Chunmei Yin;Min Ma;Ying Zhou;Xiaoyun Zheng;Chuanfu An;Yuda Fang
生长素是一种重要的植物激素，参与调节大多数植物的生长和发育反应。特别是，表观遗传机制，如组蛋白修饰和DNA甲基化，据报道影响生长素的生物合成和运输。然而，其他表观遗传因素（例如组蛋白变体H2A.Z）参与生长素相关的发育调节仍不清楚。我们报道了拟南芥Col-0生态型中的组蛋白变体H2 A. Z敲除突变体h2 a.z-kd具有更多的侧根和与生长素调节的生长性能相关的弱重力响应。进一步的研究表明，生长素促进H2A.Z从生长素应答基因SMALL AUXIN-UP RNA（SAURs）中驱逐，以激活其转录。我们发现IAA通过同源框蛋白22/25（AtHB 22/25）转录因子促进H2 A. Z基因的转录，所述转录因子在生长素信号传导中作为ARF 7/19的下游靶点起作用。双突变体hb 22和hb 25表现出与h2 a相似的侧根和向重力性表型。我们的研究结果揭示了一个相互调节枢纽，通过INOSITOL AUXOTROPHY 80介导的H2A.Z驱逐和ARF 7/19-HB 22/25介导的H2A.Z转录来调节拟南芥中SAURs的激活和植物生长。
Auxin is a critical phytohormone that is involved in the regulation of most plant growth and developmental responses. In particular, epigenetic mechanisms, like histone modifications and DNA methylation, were reported to affect auxin biosynthesis and transport. However, the involvement of other epigenetic factors, such as histone variant H2A.Z, in the auxin-related developmental regulation remains unclear. We report that the histone variant H2A.Z knockdown mutant in Arabidopsis Col-0 ecotype, h2a.z-kd, has more lateral roots and weak gravitational responses related to auxin-regulated growth performances. Further study revealed that auxin promotes the eviction of H2A.Z from the auxin-responsive genes SMALL AUXIN-UP RNAs (SAURs) to activate their transcriptions. We found that IAA promotes the transcription of H2A.Z genes through HOMEOBOX PROTEIN 22/25 (AtHB22/25) transcription factors which work as downstream targets of ARF7/19 in auxin signaling. Double mutant of hb22 hb25 showed similar lateral root and gravitropism phenotypes to h2a.z-kd. Our results shed light on a reciprocal regulation hub through INOSITOL AUXOTROPHY 80-mediated H2A.Z eviction and ARF7/19-HB22/25-mediated H2A.Z transcription to modulate the activation of SAURs and plant growth in Arabidopsis.