Conditioned medium of olfactory ensheathing cells promotes the functional recovery and axonal regeneration after contusive spinal cord injury
Conditioned medium of olfactory ensheathing cells promotes the functional recovery and axonal regeneration after contusive spinal cord injury
复制标题
嗅鞘细胞条件培养基促进脊髓挫伤后功能恢复和轴突再生
DOI：
10.1016/j.brainres.2016.10.023
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发表时间：
2017-01-01
期刊：
影响因子：
2.9
通讯作者：
He, Xijing
中科院分区：
文献类型：
--
作者：
Gu, Mengchao;Gao, Zhengchao;He, Xijing
目的：目的：探讨嗅鞘细胞条件培养液（OEC-M）治疗脊髓挫伤的有效性和潜在作用。雄性Sprague-Dawley大鼠腹腔注射对照培养基（Con-M，n=18）、低剂量OEC-M（n=18）和高剂量OEC-M（n=18）。采用免疫荧光法鉴定培养的嗅鞘细胞的纯度。采用BBB评分评价功能恢复情况。ELISA法测定OEC-M、Con-M和大鼠血浆中BDNF的浓度。采用常规MR 1和弥散张量纤维束成像（DTT）观察脊髓损伤修复过程。结果：与Con-M组相比，OEC-M组大鼠BBB评分和外周血BDNF水平均显著升高（P < 0. 05），而Con-M组大鼠BBB评分和外周血BDNF水平均显著升高（P < 0. 05）。MRI和DTT显示OEC-M治疗组SCI后放射学恢复较好，且OEC-M剂量越高，预后越好。结论：OEC-M能促进脊髓损伤后功能恢复，促进损伤中心周围的轴突再生。这种策略代表了一种新的基于无细胞OEC的疗法，以有效治疗SCI。
Objective: To investigate the effectiveness and the potential role of olfactory ensheathing cell conditioned medium (OEC-M) to treat contusive spinal cord injury (SCI).Methods: The contusive SCI model was established by NYU weight-drop impactor at T10 segment. Male Sprague-Dawley rats received intraperitoneal injection of control medium (Con-M, n=18), low dose OEC-M (n=18) and high dose OEC-M (n=18). Immunofluorescence was performed to identify the purity of cultured OECs. BBB score was used to assess the functional recovery. BDNF concentrations of OEC-M, Con-M and rats plasma samples were determined by ELISA. Conventional MR1 and diffusion tensor tractography (DTT) were applied to visualize the repair process of SCI. Diffusion tensor imaging (DTI) and immunohistochemistry of NF200 were carried out to evaluate the axonal regeneration.Results: After the systemic delivery of treatment, BBB scores and BDNF levels in peripheral blood were significantly higher in OEC-M treated groups than in Con-M group. MRI and DTT demonstrated a better radiological recovery after SCI in OEC-M treated groups and higher dose of OEC-M were accompanied with better outcomes. DTI and NF200 staining revealed the OEC-M treatment promoted axonal regeneration around the injury epicenter but no axonal regeneration was observed at the lesion site.Conclusion: The results indicate that the OEC-M treatment improves the functional recovery and promotes the axonal regeneration around the injury epicenter after contusive SCI. This strategy represents a novel cell-free OEC based therapy to effectively treat SCI.