A medaka gene map: The trace of ancestral vertebrate proto-chromosomes revealed by comparative gene mapping
A medaka gene map: The trace of ancestral vertebrate proto-chromosomes revealed by comparative gene mapping
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DOI：
10.1101/gr.2004004
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发表时间：
2004-05-01
期刊：
影响因子：
7
通讯作者：
Mitani, H
中科院分区：
文献类型：
--
作者：
Naruse, K;Tanaka, M;Mitani, H
斑马鱼中Hox簇和许多重复基因的定位表明，在鳍鱼中存在额外的全基因组重复。然而，为了重建预复制染色体（原染色体），更远的关系的硬骨鱼类的比较基因组研究是必要的。青鳉鱼和斑马鱼是理想的，因为它们的谱系在1.4亿年前与它们最后的共同祖先分离。为了重建古老的脊椎动物染色体，包括4.5亿年前人类脊椎动物祖先的染色体，我们绘制了818个基因和表达序列标签（EST）的一个单一的减数分裂回交面板上获得的近交系的青鳉，青鳉。通过比较青鳉、斑马鱼和人类3种脊椎动物的正向基因连锁关系，发现鳍鱼和叶鳍鱼（包括人类）的最后共同祖先的染色体数目和含量相同，鳍鱼谱系中的额外全基因组复制事件可能发生在所有硬骨鱼的共同祖先中。
The mapping of Hox clusters and many duplicated genes in zebrafish indicated an extra whole-genome duplication in ray-fined fish. However, to reconstruct the preduplication chromosomes (proto-chromosomes), the comparative genomic studies of more distantly related teleosts are essential. Medaka and zebrafish are ideal for this purpose, because their lineages separated from their last common ancestor similar to140 million years ago. To reconstruct ancient vertebrate chromosomes, including the chromosomes of the vertebrate ancestor of humans from 450 million years ago, we mapped 818 genes and expressed sequence tags (ESTs) on a single meiotic backcross panel obtained from inbred strains of the medaka, Oryzias latipes. Comparisons of linkage relationships of orthologous genes among three species of vertebrates (medaka, zebrafish, and human) indicate the number and content of the chromosomes of the last common ancestor of ray-fined fish and lobe-fined fish (including humans), and the extra whole genome duplication event in the ray-fin lineage occurred in the common ancestor of perhaps all teleosts.