Multi-layered Channel Patterning by Local Heating of Hydrogels
Multi-layered Channel Patterning by Local Heating of Hydrogels
复制标题
通过水凝胶局部加热形成多层通道图案
DOI：
10.1109/lra.2017.2655625
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发表时间：
2017
影响因子：
5.2
通讯作者：
T. Fukuda
中科院分区：
文献类型：
--
作者：
M. Takeuchi;T. Oya;A. Ichikawa;A. Hasegawa;M. Nakajima;Y. Hasegawa;T. Fukuda
在这封信中，我们进行了一种新的方法来制造细胞结构内的通道，用于组织工程应用。将细胞包埋的水凝胶局部熔化以在细胞结构内制造通道。制造的通道可以用作血管样网络，为细胞提供营养和氧气，或在细胞结构内形成另一种类型的细胞。采用光刻技术在玻璃衬底上制备了微电极作为加热器。使用微电极进行通道图案化。当施加到微电极上的电压恒定时，通道的宽度和高度由加热持续时间控制。通过重复熔融-填充水凝胶来实现多层通道的制造。使用肝细胞和成纤维细胞进行水凝胶中不同细胞类型的图案化。所提出的方法可以在细胞结构中制造血管样通道，以实现体外3-D共培养细胞系统。
In this letter, we conducted a new method to fabricate channels inside cell structures for tissue engineering applications. A cell-embedded hydrogel is locally melted to fabricate channels inside cell structures. The fabricated channels can be used as vascular-like networks to supply nutrition and oxygen to cells, or patterning another types of cells inside the cell structures. Microelectrodes were prepared as heaters on a glass substrate by photolithography techniques. The channel patterning was conducted using the microelectrodes. The channel width and height are controlled by the heating duration when the applied voltage to the microelectrodes is constant. The multi-layered channel fabrication was achieved by repeating melting-filling hydrogel. Patterning of different cell types in hydrogel was conducted using liver cells and fibroblast cells. The proposed method can fabricate vascular-like channels in cell structures to achieve in vitro 3-D co-culture cell systems.