Ultrabright Fluorescent Silica Mesoporous Silica Nanoparticles: Control of Particle Size and Dye Loading
Ultrabright Fluorescent Silica Mesoporous Silica Nanoparticles: Control of Particle Size and Dye Loading
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DOI：
10.1002/adfm.201100311
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发表时间：
2011-08-23
影响因子：
19
通讯作者：
Sokolov, Igor
中科院分区：
文献类型：
--
作者：
Cho, Eun-Bum;Volkov, Dmytro O.;Sokolov, Igor
本文报道了负载不同荧光染料（罗丹明6 G）的不同尺寸的超亮荧光介孔二氧化硅纳米粒子（UFSNPs）的合成。染料被物理地截留在模板化溶胶-凝胶自组装过程中产生的二氧化硅基质的纳米通道内。由于特定的纳米环境，包封的染料分子的荧光直到非常高的浓度仍保持未淬灭，这导致相对高的荧光。颗粒尺寸（范围为20-50 nm）和染料负载量（0.8-9.3 mg染料/g颗粒）通过合成的时间和几种有机三乙氧基硅烷的浓度来控制，所述有机三乙氧基硅烷是二氧化硅的共前体。包封染料的量子产率范围为0.65至1.0。单个颗粒的相对亮度相当于水中30-770个游离非二聚R6 G染料分子的荧光，或相当于1.5-39个CdSe/ZnS量子点的荧光。尽管颗粒表面存在一些疏水基团，但颗粒的胶体悬浮液相对稳定（监测120天）。
The synthesis of ultrabright fluorescent mesoporous silica nanoparticles (UFSNPs) of various sizes loaded with different amounts of fluorescent dye (Rhodamine 6G) is reported here. The dye is physically entrapped inside the nanochannels of the silica matrix created during templated sol-gel self assembly. Due to the specific nanoenvironment, the fluorescence of the encapsulated dye molecules remains unquenched up to very high concentrations, which results in relatively high fluorescence. The particle size (ranging from 20-50 nm) and dye loading (0.8-9.3 mg dye per g particles) are controlled by the timing of the synthesis and the concentration of several organ-otriethoxysilanes, which are coprecursors of silica. The quantum yields of the encapsulated dye range from 0.65 to 1.0. The relative brightness of a single particle is equivalent to the fluorescence of 30-770 free nondimerized R6G dye molecules in water, or to that of 1.5-39 CdSe/ZnS quantum dots. Despite the presence of some hydrophobic groups on the particles' surfaces, colloidal suspensions of the particles are relatively stable (as monitored for 120 days).