Tryptophan micro-scale determinations by rapid hydrolysis.
Tryptophan micro-scale determinations by rapid hydrolysis.
复制标题
通过快速水解进行微量色氨酸测定。
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
A. Tsugita
中科院分区：
文献类型：
--
作者：
K. Maeda;J. Scheffler;A. Tsugita
建立了快速测定蛋白质中色氨酸含量的酸水解微法。样品蛋白（5微克）在30微升3M巯基乙磺酸中，在166℃下水解25分钟，导致肽键完全水解。水解产物经氨基酸分析仪测定氨基酸组成，包括色氨酸的含量，回收率高达92%。
A rapid acid-hydrolysis micro method was developed for the accurate determination of the tryptophan contents of proteins. Sample protein (5 micrograms) was hydrolysed in 30 microliters of 3M mercaptoethanesulfonic acid at 166 degrees C for 25 min, resulting in total hydrolysis of the peptide bonds. The subjection of the hydrolysate to an amino-acid analyser revealed the amino-acid composition, including the content of tryptophan with a high recovery of 92%.