Discordance between surface and cytoplasmic expression of the Leu-4 (T3) antigen in thymocytes and in blast cells from childhood T lymphoblastic malignancies.
Discordance between surface and cytoplasmic expression of the Leu-4 (T3) antigen in thymocytes and in blast cells from childhood T lymphoblastic malignancies.
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儿童 T 淋巴细胞恶性肿瘤的胸腺细胞和母细胞中 Leu-4 (T3) 抗原的表面和细胞质表达之间的不一致。
DOI：
10.1172/jci111954
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Levy,R
中科院分区：
文献类型：
--
作者：
Link,MP;Stewart,SJ;Warnke,RA;Levy,R
我们检测了23例T细胞急性淋巴细胞白血病和T细胞淋巴细胞淋巴瘤母细胞表面和胞浆中Leu-4（T3）抗原的表达。在大多数病例中（17例），Leu-4抗原在细胞表面不存在;然而，在这17例病例中的16例中，母细胞显示Leu-4的胞质表达。Leu-4的表面和细胞质表达之间的这种不一致也在胸腺细胞中发现，并且似乎仅限于Leu-4，因为其他T细胞抗原的测试很少显示表面和细胞质表达之间的不一致。为了进一步研究在恶性T淋巴母细胞中通过抗Leu-4鉴定的细胞质决定簇，进行免疫沉淀研究，其利用来自T淋巴母细胞恶性肿瘤的已建立的T细胞系的生物合成标记。通过一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，从表面Leu-4+和表面Leu-4-/胞质Leu-4+细胞系中免疫沉淀出相同的Leu-4多肽家族。因为T淋巴母细胞恶性肿瘤代表未成熟T细胞的增殖，并且因为所研究的病例证明了对应于T细胞个体发育的所有拟议阶段的表面表型，所以Leu-4的细胞质表达似乎发生在T细胞发育的早期。这些未成熟T细胞无法将Leu-4分子转运到其表面的原因仍有待阐明。图片
We have examined the expression of the Leu-4 (T3) antigen on the cell surface and in the cytoplasm of blast cells from 23 patients with T cell acute lymphoblastic leukemia and T cell lymphoblastic lymphoma. In the majority of cases (17), the Leu-4 antigen was absent from the cell surface; however, in 16 of these 17 cases, blast cells demonstrated cytoplasmic expression of Leu-4. This discordance between surface and cytoplasmic expression of Leu-4 was also found in thymocytes and appeared to be restricted to Leu-4, in that tests of other T cell antigens rarely revealed discordance between surface and cytoplasmic expression. To study further the cytoplasmic determinant identified by anti-Leu-4 in malignant T lymphoblasts, immunoprecipitation studies were performed that utilized biosynthetic labeling of established T cell lines derived from T lymphoblastic malignancies. By one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, identical Leu-4 polypeptide families were immunoprecipitated from surface Leu-4+ and surface Leu-4-/cytoplasmic Leu-4+ cell lines. Because T lymphoblastic malignancies represent proliferations of immature T cells, and because the cases studied demonstrated surface phenotypes corresponding to all of the proposed stages of T cell ontogeny, it appears that cytoplasmic expression of Leu-4 occurs early in T cell development. The reason for the failure of these immature T cells to transport the Leu-4 molecule to their surface remains to be elucidated.Images
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细胞表面抗原：儿童急性淋巴细胞白血病的预后意义。
影响因子：
20.3
作者：
S. Sallan;J. Ritz;J. Pesando;R. Gelber;C. O'Brien;S. Hitchcock;F. Coral;S. Schlossman
通讯作者：
S. Schlossman
人类胸腺依赖性膜抗原：TH2 抗原单克隆抗体抑制细胞介导的淋巴溶解。
DOI：
10.1073/pnas.78.1.544
发表时间：
1981
影响因子：
11.1
作者：
R. Evans;D. Wall;C. Platsoucas;F. Siegal;S. Fikrig;C. Testa;R. Good
通讯作者：
R. Good
通过流式细胞术检测胞浆内抗原。
影响因子：
2.2
作者：
Schroff,RW;Bucana,CD;Klein,RA;Farrell,MM;MorganJr,AC
通讯作者：
MorganJr,AC
人类 T 细胞白血病的衍生。
影响因子：
11.2
作者：
Reinherz,EL;Schlossman,SF
通讯作者：
Schlossman,SF
非T细胞急性淋巴细胞白血病的B细胞起源。
DOI：
10.1172/jci111428
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Nadler,LM;Korsmeyer,SJ;Anderson,KC;Boyd,AW;Slaughenhoupt,B;Park,E;Jensen,J;Coral,F;Mayer,RJ;Sallan,SE
通讯作者：
Sallan,SE