Intermittent fasting increases energy expenditure and promotes adipose tissue browning in mice
Intermittent fasting increases energy expenditure and promotes adipose tissue browning in mice
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DOI：
10.1016/j.nut.2019.03.015
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发表时间：
2019-10-01
期刊：
影响因子：
4.4
通讯作者：
Heilbronn, Leonie K.
中科院分区：
文献类型：
--
作者：
Liu, Bo;Page, Amanda J.;Heilbronn, Leonie K.
目的：间歇性禁食（IF）可能通过刺激白色脂肪组织（WAT）布朗宁来限制降低能量消耗的代谢适应。本研究的目的是检查8周的IF对能量代谢和标记物的WAT布朗宁在瘦和饮食诱导的肥胖小鼠和妇女谁是超重或obesity.Methods：雄性C57 BL/6 J小鼠喂养的食物或高脂饮食（HFD; 43%）8周前进行IF（3个非连续d/周）的额外8周。在进食或禁食条件下收集食物摄入、能量消耗以及腹股沟和性腺脂肪垫（22 h，仅IF小鼠）。皮下脂肪组织（SAT）也收集了基线，8周后的IF（在进食状态下，24小时禁食后），超重或肥胖的妇女。解偶联蛋白1（UCP 1）进行了评估，通过定量实时聚合酶链反应（小鼠和人类）和免疫组织化学（小鼠）。结果：IF降低体重和能量摄入的HFD喂养的小鼠和减少性腺和腹股沟脂肪垫的重量在两个饮食组。IF增加能量消耗，餐数，UCP 1 mRNA水平在腹股沟和性腺脂肪库，和UCP 1蛋白在腹股沟脂肪组在喂养日。在妇女，IF体重和脂肪量减少，但没有改变UCP 1 mRNA水平。结论：IF增加能量消耗，促进WAT布朗宁小鼠，但没有改变UCP 1 mRNA水平在SAT的妇女。（C）2019 Elsevier Inc. All rights reserved.
Objective: Intermittent fasting (IF) may limit metabolic adaptations that reduce energy expenditure, potentially by stimulating white adipose tissue (WAT) browning. The aim of this study was to examine the effects of 8 wk of IF on energy metabolism and markers of WAT browning in lean and diet-induced obese mice and in women who were overweight or obese.Methods: Male C57 BL/6 J mice were fed chow or a high-fat diet (HFD; 43%) for 8 wk before undergoing IF (3 non-consecutive d/wk) for an additional 8 wk. Food intake, energy expenditure, and inguinal and gonadal fat pads were collected in fed or fasted conditions (22 h, IF mice only). Subcutaneous adipose tissue (SAT) was also collected at baseline, and after 8 wk of IF (in the fed state, and after a 24-h fast), in women with overweight or obesity. Uncoupling protein 1 (UCP1) was assessed by quantitative real-time polymerase chain reaction (mice and humans) and immunohistochemistry (mice).Results: IF reduced body weight and energy intake in HFD fed mice and reduced gonadal and inguinal fat pad weights in both diet groups. IF increased energy expenditure, meal number, Ucp1 mRNA levels in inguinal and gonadal fat depots, and UCP1 protein in inguinal fat in both diet groups on fed days. In women, IF reduced body weight and fat mass, but did not alter UCP1 mRNA levels.Conclusions: IF increased energy expenditure and promoted WAT browning in mice but did not alter UCP1 mRNA levels in SAT in women. (C)2019 Elsevier Inc. All rights reserved.