Distribution of zinc and zinc transporters in the mouse ovarian follicles and corpus luteum.
Distribution of zinc and zinc transporters in the mouse ovarian follicles and corpus luteum.
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DOI：
10.14670/hh-28.1517
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发表时间：
2013-05
影响因子：
2
通讯作者：
Manli Zhong;Chuang Guo;Zhi-hong Chi;Z. Shan;W. Teng;Zhan-You Wang
中科院分区：
文献类型：
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作者：
Manli Zhong;Chuang Guo;Zhi-hong Chi;Z. Shan;W. Teng;Zhan-You Wang
锌对女性生殖至关重要，它在性发育、排卵、月经和发情周期中起着重要作用。缺锌可能导致女性生殖系统功能障碍。本研究旨在通过锌自金相法（AMG）、免疫组化和实时荧光定量PCR （real-time PCR）技术，研究游离锌和锌转运蛋白家族成员（ZnT）的表达和分布规律，探讨锌在小鼠卵巢发育和功能中的动态平衡关系。我们的数据显示，游离锌离子和znt主要分布在小鼠卵巢卵泡和黄体中。具体来说，AMG染色出现在卵巢卵泡和黄体的各个阶段。ZnT1-9 mRNA在卵巢中有不同程度的表达，而ZnT10 mRNA在卵巢中几乎检测不到。此外，除ZnT10外，所有被测znt在小鼠原始卵泡、初级卵泡、次级卵泡和窦泡中均具有不同强度的免疫反应性。在黄体中，ZnT1-5、7、8、10在颗粒状和膜状叶黄素细胞和间质细胞中大量出现免疫反应性。综上所述，我们的研究结果表明，ZnT家族蛋白在控制细胞锌水平方面分布不同，并可能发挥不同的生物学功能，从而调节小鼠卵巢的发育和功能。
Zinc is essential for female reproduction and it plays a role in sexual development, ovulation, menstruation and estrous cycles. Zinc deficiency may lead to female reproductive system dysfunction. The present study aimed to investigate the expression and distribution patterns of free zinc and the members of zinc transporter (ZnT) family, with zinc autometallographic (AMG), immunohistochemistry and real-time PCR, to explore the relationship of zinc homeostasis in the development and function of the ovary in the mouse. Our data revealed that the free zinc ions and ZnTs are predominantly distributed in the mouse ovarian follicles and corpus luteum. Specifically, AMG staining presented in various stages of the ovarian follicles and corpus luteum. ZnT1-9 mRNA was variously expressed, whereas ZnT10 mRNA was almost undetectable in the ovary. Moreover, the immunoreactivity of all the tested ZnTs, except for ZnT10, was detected with various intensity in the mouse primordial follicles, primary follicles, secondary follicles and antral follicles. In the corpus luteum, the immunoreactivity of ZnT1-5, 7, 8, 10, was abundantly observed in the granular and theca lutein cells and interstitial cells. Collectively, our results suggest that ZnT family proteins are differently distributed and might exert different biological functions in controlling cellular zinc levels, which regulate ovarian development and function in the mouse ovary.