Seasonality in bacterial diversity in north-west Mediterranean coastal waters:: assessment through clone libraries, fingerprinting and FISH
Seasonality in bacterial diversity in north-west Mediterranean coastal waters:: assessment through clone libraries, fingerprinting and FISH
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DOI：
10.1111/j.1574-6941.2006.00276.x
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发表时间：
2007-04-01
影响因子：
4.2
通讯作者：
Gasol, Josep M.
中科院分区：
文献类型：
--
作者：
Alonso-Saez, Laura;Balague, Vanessa;Gasol, Josep M.
采用变性梯度凝胶电泳（DGGE）、催化报告沉积- fish （CARD-FISH）和克隆文库等方法，对地中海西北部沿海海域细菌组合组成的季节性进行了研究。DGGE分析表明，全年细菌多样性逐渐变化，但夏季差异明显。在年度基础上，Alphaproteobacteria是优势群体[29%的CARD-FISH DAPI（4′，6-二氨基-2-苯基吲哚）计数，70%的细菌克隆]。SAR11分支在春季和夏季最丰富（占DAPI计数的20%），而玫瑰杆菌分支主要在冬季和春季丰富（占DAPI计数的7%）。拟杆菌门是第二重要的类群，其数量全年一致（平均占DAPI计数的11%）。γ变形菌属在夏季达到峰值（占DAPI计数的8%），大多数属于NOR5簇。克隆文库与CARD-FISH在拟杆菌门（Bacteroidetes）和γ变形菌门（Gammaproteobacteria）的数量比例上基本一致，但α变形菌门（Alphaproteobacteria）在克隆文库中的数量比例过高。尽管SAR11的丰度很高，但对最主要的DGGE条带的测序未能检测到SAR11组。这三种分子方法的结合可以全面评估细菌多样性的季节性变化。
We combined denaturing gradient gel electrophoresis (DGGE), catalysed reporter deposition-FISH (CARD-FISH) and clone libraries to investigate the seasonality of the bacterial assemblage composition in north-west Mediterranean coastal waters. DGGE analysis indicated that bacterial diversity changed gradually throughout the year, although with a clear distinction of the summer period. Alphaproteobacteria were the dominant group on an annual basis [29% of the DAPI (4',6-diamidino-2-phenylindole) counts by CARD-FISH, and 70% of the bacterial clones]. The SAR11 clade was most abundant during spring and summer (> 20% of DAPI counts), while the Roseobacter clade was abundant primarily in winter and spring (up to 7% of DAPI counts). The phylum Bacteroidetes constituted the second most important group and was quantitatively uniform throughout the year (average 11% of the DAPI counts). Gammaproteobacteria showed a peak during summer (8% of DAPI counts), when most of them belonged to the NOR5 cluster. Clone libraries and CARD-FISH showed reasonable agreement in the quantitative proportions of Bacteroidetes and Gammaproteobacteria, but Alphaproteobacteria were overrepresented in clone libraries. Sequencing of the most predominant DGGE bands failed to detect the SAR11 group despite their high abundance. The combination of the three molecular approaches allowed a comprehensive assessment of seasonal changes in bacterial diversity.