The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.
The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.
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DOI：
10.17912/micropub.biology.000593
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发表时间：
2022
影响因子：
--
通讯作者：
Miller, Adam C
中科院分区：
文献类型：
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作者：
Michel, Jennifer Carlisle;Lasseigne, Abagael M;Marsh, Audrey J;Miller, Adam C
为了研究体内电突触的形成，我们使用正向遗传学来破坏影响幼斑马鱼Mauthner细胞电突触的基因。我们确定disconnect2（dis2）突变的失败，本地化神经间隙连接通道在电突触。我们将这种突变定位在25号染色体上，并在tjp1b基因中发现了一个剪接改变突变。我们使用CRISPR产生的突变体进行互补分析，证明了dis2突变破坏了tjp1b功能。我们的结论是dis2突变破坏了tjp1b基因，这是电突触形成所必需的。
To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this mutation to chromosome 25 and identified a splice-altering mutation in the tjp1b gene. We demonstrated that the dis2 mutation disrupts tjp1b function using complementation analysis with CRISPR generated mutants. We conclude that the dis2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.