Survival factor-insensitive generation of reactive oxygen species induced by serum deprivation in neuronal cells
Survival factor-insensitive generation of reactive oxygen species induced by serum deprivation in neuronal cells
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DOI：
10.1016/s0006-8993(96)00527-6
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发表时间：
1996-09-09
期刊：
影响因子：
2.9
通讯作者：
Hatanaka, H
中科院分区：
文献类型：
--
作者：
Satoh, T;Sakai, N;Hatanaka, H
为了研究活性氧（ROS）在神经元凋亡中的作用，我们用ROS特异性荧光团6-羧基-2 '，7'-二氯二氢荧光素二乙酸酯二乙酰氧基甲酯（C-DCDHF-DA）进行了共聚焦和流式细胞术分析。血清剥夺显著增加PC 12细胞和大鼠皮层神经元的ROS水平。抗氧化剂N，N '-二苯基-对苯二胺（DPPD）可减少血清剥夺诱导的ROS产生并恢复细胞存活。而神经生长因子、Bcl-2等抑制PC 12细胞凋亡的生存因子对血清剥夺诱导的ROS上调无影响。表皮生长因子可抑制皮层神经元的凋亡，但不影响ROS的增加。这些数据表明，生存因子拯救血清剥夺诱导的细胞凋亡独立的ROS的生产。
To investigate the involvement of reactive oxygen species (ROS) in neuronal apoptosis, we performed confocal and flow cytometric analysis with a ROS-specific fluorogen, 6-carboxy-2',7'-dichorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA). Serum deprivation significantly increased the level of ROS in PC12 cells and rat cortical neurons. N,N'-diphenyl-p-phenylenediamine (DPPD), an antioxidant, reduced ROS production induced by serum deprivation and recovered cell survival. However, some survival factors such as nerve growth factor and Bcl-2, which prevented the apoptosis of PC12 cells, did not affect the up-regulation of ROS induced by serum deprivation. Epidermal growth factor which prevented the apoptosis of cortical neurons, did not affect the increase of ROS. These data suggest that survival factors rescue the serum deprivation-induced apoptosis independently of ROS production.