Assignment of phosphorus-31 and nonexchangeable proton resonances in a symmetrical 14 base pair lac pseudooperator DNA fragment.
Assignment of phosphorus-31 and nonexchangeable proton resonances in a symmetrical 14 base pair lac pseudooperator DNA fragment.
复制标题
对称 14 碱基对 lac 伪操作子 DNA 片段中磷 31 和不可交换质子共振的分配。
DOI：
10.1021/bi00387a012
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Gorenstein,DG
中科院分区：
文献类型：
--
作者：
Schroeder,SA;Fu,JM;Jones,CR;Gorenstein,DG
斯蒂芬·A·施罗德，约瑟芬·M·傅，克劳德·R·琼斯和大卫·G·戈伦斯坦*普渡大学化学系，西拉斐特，印第安纳州47907，收到1986年10月10日；修订手稿收到1987年1月14日摘要：所有13种磷酸盐的31P化学位移和对称的14碱基对Lac假操作符DNA片段的几乎所有不可交换质子的化学位移已通过区域特异性标记和氧-17标记和二维核磁共振技术进行了归属。在22℃时，13个磷共振中的8个可以明显分辨，而其余5个共振发生在两个独立的重叠区域。正如其他31P分配研究所显示的那样，这种特殊的14碱基对寡核苷酸的31P化学位移与一般观察到的情况不符，即磷酸在寡核苷酸序列中的内部越多，31P共振发生的越多。根据Callidine的分析[Callidine，CR(1982)J.Mol]，这一一般规则的失败被认为是沿着寡核苷酸双螺旋发生螺旋扭曲的结果。比奥尔。161,343-352]，磷酸盐位置关系的显著例外是5‘-Py-Pu-3’二核苷酸序列，它们在比预期更低的场强下共振，与Ott和Eackstein报道的类似结果一致[Ott，J.，&
Stephen A. Schroeder, Josepha M. Fu, Claude R. Jones, and David G. Gorenstein* Department of Chemistry, Purdue University, West Lafayette, Indiana 47907 Received October 10, 1986; Revised Manuscript Received January 14, 1987 abstract: The 31P chemical shifts of all 13 phosphates and the chemical shifts of nearly all of the non-exchangeable protons of a symmetrical 14 base pair lac pseudooperator DNA fragment have been assigned by regiospecific labelingwith oxygen-17 and two-dimensional NMR techniques. At 22 C, 8 of the 13 phosphorus resonances can distinctly be resolved while the remaining 5 resonances occur in two separate overlapping regions. The 31P chemical shifts of this particular 14 base pair oligonucleotide do not follow the general observation that the more internal thephosphate is located within the oligonucleotide sequence the more upfield the 31P resonance occurs, as shown from other 31P assignment studies. Failure of this general rule is believed to be a result of helical distortions that occur along the oligonucleotide double helix, on the basis of the analysis of Callidine [Callidine, CR (1982) J. Mol. Biol. 161, 343-352], Notable exceptions to the phosphate position relationship are 5'-Py-Pu-3'dinucleotide sequences, which resonate at a lower field strength than expected in agreement with similar results as reported by Ott and Eckstein [Ott, J., &