Enhancing Antioxidant Performance of Lignin by Enzymatic Treatment with Laccase
Enhancing Antioxidant Performance of Lignin by Enzymatic Treatment with Laccase
复制标题
漆酶酶处理增强木质素的抗氧化性能
DOI：
10.1021/acssuschemeng.7b04070
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发表时间：
2018-02-01
影响因子：
8.4
通讯作者：
Ge, Yuanyuan
中科院分区：
文献类型：
--
作者：
Li, Zhili;Zhang, Jiubing;Ge, Yuanyuan
The utilization of lignin is of high interest since it represents the most abundant polyphenol worldwide. For the application of lignin as a natural antioxidant more phenolic hydroxyl groups (Ph-OH) and lower molecular weight (M-w) are essential. Here, we proposed a green method based on enzymatic treatment of two lignins (alkaline lignin AL and hydrolysis lignin HL) with laccase, by which the structure, especially the Ph-OH contents and M-w of lignin, could effectively controlled. The antioxidant activity of the lignin was evaluated in vitro by DPPH center dot (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability. The mechanism of antioxidant activity improvement was also elucidated. As expected, the enzymatic treated lignin (HL-L1.0) with the highest Ph-OH content and lower M-w exhibited the strongest antioxidant activity (IC50 = 28.8 mu g/mL), which was even stronger than the commercial antioxidants BHT (3,5-di-tert-butyl-4-hydroxytoluene, IC50 = 38.2 mu g/mL) and BHA (3-tert-butyl-4-hydroxyanisole, IC50 = 56.3 mu g/mL). This study suggested that the enzymatic treatment was a green and effective way to obtain lignin derivatives that showed great potential for antioxidant applications.
