Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice.
Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice.
复制标题
接触新奇事物对 C57BL/6 和 DBA/2 小鼠脑单胺的影响。
DOI：
10.1016/0031-9384(95)02010-1
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发表时间：
1996
影响因子：
2.9
通讯作者：
Cook,MN
中科院分区：
文献类型：
--
作者：
Jones,BC;Hou,X;Cook,MN
将来自两个近交系 C57BL/6 (B6) 和 DBA/2 (D2) 的雄性和雌性小鼠暴露于新环境（相对于未受干扰的对照）10 分钟。处理后立即通过颈脱位处死动物，取出大脑并解剖成腹侧中脑（VMB）、尾壳核（CP）、伏隔核（NA）和内侧前额皮质（FC）。采用高效液相色谱法对多巴胺 (DA) 及其代谢物二羟基苯乙酸 (DOPAC)、高香草酸 (HVA) 和血清素 (5-HT) 及其代谢物 5-羟基吲哚-3-乙酸 (5-HIAA) 进行分析。母体胺的利用率通过比率DOPAC/DA、HVA/DA 和5-HIAA/5-HT 来估计。新颖性增加了两种小鼠品系的 NA 和 D2 雄性小鼠的 CP 中的 DOPAC 水平；然而，它并没有引起 DA 或 5-HT 水平或后者利用率的显着变化。然而，结果确实揭示了 DA 及其代谢物的巨大菌株差异。数据表明，小鼠的多巴胺能和血清素能系统中存在基于遗传的神经生理学和神经化学差异，并且 NA 中的 DA 系统可能比其他 DA 系统对新奇事物更敏感。
Male and female mice from two inbred strains, C57BL/6 (B6) and DBA/ 2 (D2), were exposed to a novel environment (vs. undisturbed control) for 10 min. Immediately after this treatment, the animals were sacrificed by cervical dislocation, and the brains were removed and dissected into ventral midbrain (VMB), caudate-putamen (CP), nucleus accumbens (NA), and medial prefrontal cortex (FC). Analyses of dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and serotonin (5-HT) and its metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) were made by high-performance liquid chromatography. Utilization of the parent amines was estimated by the ratios, DOPAC/DA, HVA/DA, and 5-HIAA/5-HT. Novelty increased DOPAC levels in NA of both strains of mice and in CP of D2 males; however, it did not induce significant changes in DA, or 5-HT levels or utilization of the latter. The results did, however, reveal large strain differences in DA and its metabolites. The data suggest that genetically based neurophysiological and neurochemical differences exist in dopaminergic and serotonergic systems in mice, and that the DA systems in NA may be more sensitive to novelty than other DA systems.