Hyperspectral phasor analysis enables multi-plexed 5D in vivo imaging
Hyperspectral phasor analysis enables multi-plexed 5D in vivo imaging
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DOI：
10.1038/nmeth.4134
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发表时间：
2017-02-01
期刊：
影响因子：
48
通讯作者：
Fraser, Scott E.
中科院分区：
文献类型：
--
作者：
Cutrale, Francesco;Trivedi, Vikas;Fraser, Scott E.
多个标记的延时成像对于生物成像是具有挑战性的，因为噪声、光漂白和光毒性损害信号质量，而处理量可能受到处理时间的限制。在这里，我们报告了一种名为超光谱相位器（HySP）的软件，用于在低信噪比条件下对多个光谱重叠的荧光团进行去噪和解混，并进行快速分析。我们表明，HySP能够在活斑马鱼胚胎的延时成像中分解七个信号。
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.