Comparative analysis of the main active components and hypoglycemic effects after the compatibility of Scutellariae Radix and Coptidis Rhizoma
Comparative analysis of the main active components and hypoglycemic effects after the compatibility of Scutellariae Radix and Coptidis Rhizoma
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DOI：
10.1002/jssc.201801204
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发表时间：
2019-04-01
影响因子：
3.1
通讯作者：
Duan, Jin-ao
中科院分区：
文献类型：
--
作者：
Cui, Xiang;Shen, Yu-meng;Duan, Jin-ao
本研究建立了以乙腈和0.1%甲酸水溶液为移动的流动相的快速、高灵敏度的超高效液相色谱-三重四极杆质谱联用方法，并成功地应用于配伍后主要活性成分的比较分析。此外，还观察了黄芪、黄芪及其联合提取物对高脂饮食沿着低剂量链脲佐菌素诱导的2型糖尿病大鼠的影响。在优化的色谱条件下，7种目标成分在12 min内得到了良好的分离，线性关系良好（R-2 >= 0.999）。精密度、重复性和稳定性的相对标准偏差分别为0.69 ~ 2.23%、0.98 ~ 2.56%和0.92 ~ 2.57%。回收率范围为91.11 - 105.35%。配伍后，7种活性成分的含量均显著降低，但联合提取物的降血糖作用强于单药，降低了果糖-1，6-二磷酸酶、葡萄糖-6-磷酸酶、磷酸烯醇式丙酮酸羧激酶的活性，提高了葡萄糖激酶、磷酸果糖激酶、丙酮酸激酶的活性。因此，建立的分析方法是准确和灵敏度足够的定量评价的七个研究化合物。而且，该组合提取物通过多组分多靶点对2型糖尿病有明确的疗效。
In this study, a rapid and highly sensitive ultra high performance liquid chromatography with triple quadrupole mass spectrometry method with the mobile phase of acetonitrile and 0.1% aqueous formic acid was established and successfully applied to comparatively analyze main active components after their compatibility. Besides, the effects of Scutellariae Radix, Coptidis Rhizoma and combined extracts on type 2 diabetic rats induced by high-fat diet along with low dose of streptozocin were investigated. Under the optimized chromatographic conditions, good separation of seven target components was achieved within 12 min. All calibration curves exhibited good linearity (R-2 >= 0.999). The relative standard deviation of precision, repeatability and stability varied from 0.69 to 2.23, 0.98 to 2.56, and 0.92 to 2.57%, respectively. The recovery ranged from 91.11 to 105.35%. The contents of seven active components were notably reduced after compatibility; however, the hypoglycemic effect of combined extracts was stronger than single drug by decreasing the activities of fructose-1,6-bisphosphatase, glucose 6-phosphatase, phosphoenolpyruvate carboxykinase and increasing the activities of glucokinase, phosphofructokinase, pyruvate kinase. Accordingly, the established analytical method was accurate and sensitive enough for quantitative evaluation of seven investigated compounds. Moreover, the combined extract had definite effects on type 2 diabetes through multiple components against multiple targets.