Comparative proliferative and histopathologic changes in rat lungs after inhalation of chrysotile or crocidolite asbestos
Comparative proliferative and histopathologic changes in rat lungs after inhalation of chrysotile or crocidolite asbestos
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DOI：
10.1006/taap.1996.0058
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发表时间：
1996-03-01
影响因子：
3.8
通讯作者：
Mossman, BT
中科院分区：
文献类型：
--
作者：
Berube, KA;Quinlan, TR;Mossman, BT
对Fischer 344大鼠在吸入暴露于温石棉或青石棉（空气中平均浓度约为8毫克/立方米）5天和20天后，以及暴露20天后接着在室内空气中再暴露20天（20 + 20天）后，肺和胸膜中细胞增殖的模式以及肺组织病理学病变的发展进行了研究。为了评估细胞增殖，在开始暴露于石棉后的不同时间点向大鼠注射5-溴-2 '-脱氧尿苷（BrdU）。图像分析用于定量温石棉和青石棉对以下肺隔室中BrdU标记指数的影响：（1）肺隔室，（2）肺泡管区域，（3）支气管上皮，和（4）内脏间皮。除了mesothelium，其中表现出显着增加BrdU掺入大鼠暴露于青石棉在20 + 20天，石棉诱导的升高BrdU吸收在其他隔室是短暂的标记与假对照在稍后的时间点。大鼠肺组织病理学显示，在接触青石棉的大鼠中，20天后纤维化病变的范围和严重程度更大，但在清洁空气中再接触石棉20天后，两个石棉接触组均发生纤维化（20 + 20）。定量的纤维负荷在大鼠肺吸入空气中的浓度相当的纤维类型表明，青石棉的吸入导致较高的纤维保留相比，温石棉。我们的研究结果表明，温石棉和青石棉诱导肺和胸膜细胞的细胞增殖的不同模式。青石棉的间皮细胞BrdU标记的持久增加可能反映了纤维的保留增加和/或石棉类型之间的固有差异。(C)出版社：Academic Press，Inc.
Patterns of cell proliferation in lung and pleura and development of histopathologic lesions were studied in lungs from Fischer 344 rats after inhalation exposure to chrysotile or crocidolite asbestos at average airborne concentrations of approximately 8 mg/m(3) air for 5 and 20 days and after 20 days of exposure followed by an additional 20 days in room air (20 + 20 days). To assess cell proliferation rats were injected with 5-bromo-2'-deoxyuridine (BrdU) at various time points after initiation of exposure to asbestos. Image analysis was used to quantitate the effects of chrysotile and crocidolite on BrdU labeling indices in the following lung compartments: (1) interstitium, (2) alveolar duct region, (3) bronchial epithelium, and (4) visceral mesothelium. With the exception of mesothelium, which exhibited significant increases in BrdU incorporation in rats exposed to crocidolite at 20 + 20 days, asbestos-induced elevations in BrdU uptake in other compartments were transient with labeling comparable to sham controls at later time points. Histopathology of rat lungs revealed fibrotic lesions of a greater extent and severity at 20 days in rats exposed to crocidolite, but fibrosis occurred in both asbestos-exposed groups after an additional 20 days in clean air (20 + 20). Quantification of fiber burden in rat lung after inhalation of comparable airborne concentrations of either fiber type demonstrated that inhalation of crocidolite asbestos led to a higher fiber retention when compared to chrysotile asbestos. Our results indicate that chrysotile and crocidolite asbestos induce different patterns of cell proliferation in lung and pleural cells. The protracted increases in BrdU labeling of mesothelial cells by crocidolite may reflect increased retention of fibers and/or inherent differences between types of asbestos. (C) 1996 Academic Press, Inc.