Lactate Biosensor Based on the Adsorption of Polyelectrolyte Stabilized Lactate Oxidase into Porous Conductive Carbon
Lactate Biosensor Based on the Adsorption of Polyelectrolyte Stabilized Lactate Oxidase into Porous Conductive Carbon
复制标题
基于多孔导电碳吸附聚电解质稳定乳酸氧化酶的乳酸生物传感器
DOI：
10.1007/s006040170056
复制
发表时间：
2001
影响因子：
5.7
通讯作者：
N. Chaniotakis
中科院分区：
文献类型：
--
作者：
V. Gavalas;N. Chaniotakis
在这项工作中，乳酸生物传感器的发展进行了说明。乳酸氧化酶是稳定的阳离子β-二乙基氨基乙基-葡聚糖，并得到的酶-β-葡聚糖复合物被物理吸收到一个高度多孔和导电的碳电极的生物传感器的建设。使用的二乙基氨基乙基-葡聚糖的量相对于传感器灵敏度和稳定性进行优化。用含有0.5%w/v二乙基氨基乙基葡聚糖和200 U/ml乳酸氧化酶的酶溶液获得最佳结果。所得的生物传感器呈现增加的操作（超过240小时的连续极化）和储存稳定性（超过5个月），而计算的重现性优于5.0%的RSD。
In this work the development a lactate biosensor is illustrated. Lactate oxidase is stabilized with the cationic polyelectrolyte diethylaminoethyl-dextran, and the resulting enzyme-polyelectrolyte complexes are physically absorbed into a highly porous and conductive carbon electrode for the construction of the biosensor. The amount of diethylaminoethyl-dextran used is optimized with respect to the sensor sensitivity and stability. Optimum results obtained with enzyme solution containing 0.5% w/v diethylaminoethyl-dextran and 200 U/ml lactate oxidase. The resulting biosensors present increased operational (over 240 hours of continuous polarization) and storage stability (more than 5 months), while the reproducibility was calculated to be better than 5.0% RSD.