TRITICUM-AESTIVUM PUROINDOLINES, 2 BASIC CYSTINE-RICH SEED PROTEINS - CDNA SEQUENCE-ANALYSIS AND DEVELOPMENTAL GENE-EXPRESSION
TRITICUM-AESTIVUM PUROINDOLINES, 2 BASIC CYSTINE-RICH SEED PROTEINS - CDNA SEQUENCE-ANALYSIS AND DEVELOPMENTAL GENE-EXPRESSION
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DOI：
10.1007/bf00024197
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发表时间：
1994-04-01
影响因子：
5.1
通讯作者：
JOUDRIER, P
中科院分区：
文献类型：
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作者：
GAUTIER, MF;ALEMAN, ME;JOUDRIER, P
从一个成熟中期的种子cDNA文库中，我们已经分离出的cDNA克隆编码两个普通小麦puroindolines。Puroindoline-a和Puroindoline-b有55%的相似性，是碱性的、富含胱氨酸和富含色氨酸的蛋白质。Puroindolines被合成为前原蛋白，其包括可能参与其液泡定位的N-和C-末端前肽。成熟蛋白质的分子量为13 kDa，计算的等电点大于10。嘌呤二氢吲哚一级结构的一个显著特征是存在一个富含色氨酸的结构域，该结构域也含有碱性残基。在燕麦种子蛋白和哺乳动物抗菌肽中发现了类似的富含色氨酸的结构域。嘌呤吲哚啉的十个半胱氨酸残基组织在半胱氨酸骨架中，其显示出与其他小麦种子富含胱氨酸的蛋白质的半胱氨酸骨架相似。北方印迹分析表明puroindoline基因在T.发育中的种子。在硬粒小麦中未检测到puroindoline转录本及相关基因。小麦淀粉颗粒相关蛋白的嘌呤吲哚啉的身份进行了讨论，以及在植物防御机制的嘌呤吲哚啉的潜在作用。
From a mid-maturation seed cDNA library we have isolated cDNA clones encoding two Triticum aestivum puroindolines. Puroindoline-a and puroindoline-b, which are 55% similar, are basic, cystine-rich and tryptophan-rich proteins. Puroindolines are synthezised as preproproteins which include N- and C-terminal propeptides which could be involved in their vacuolar localization. The mature proteins have a molecular mass of 13 kDa and a calculated isoelectric point greater than 10. A notable feature of the primary structure of puroindolines is the presence of a tryptophan-rich domain which also contains basic residues. A similar tryptophan-rich domain was found within an oat seed protein and a mammalian antimicrobial peptide. The ten cysteine residues of puroindolines are organized in a cysteine skeleton which shows similarity to the cysteine skeleton of other wheat seed cystine-rich proteins. Northern blot analysis showed that puroindoline genes are specifically expressed in T. aestivum developing seeds. No puroindoline transcripts as well as no related genes were detected in Triticum durum. The identity of puroindolines to wheat starch-granule associated proteins is discussed as well as the potential role of puroindolines in the plant defence mechanism.