Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor
Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor
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DOI：
10.1007/s00425-011-1573-y
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发表时间：
2012-06-01
期刊：
影响因子：
4.3
通讯作者：
Huang, Shangzhi
中科院分区：
文献类型：
--
作者：
Chu, Pu;Chen, Huhui;Huang, Shangzhi
膜联蛋白是一种多功能蛋白质，其特征在于能够结合钙离子和带负电荷的脂质。虽然越来越多的证据表明它们在植物逆境应答中的重要性，但它们在种子中的功能仍有待进一步研究。在这项研究中，我们确定了一个热诱导的膜联蛋白，NnANN 1，从神圣的莲（Nelumbo nucifera Gaertn.）使用比较蛋白质组学方法。此外，NnANN 1的表达响应于高温处理而显著增加。实时荧光定量PCR（qRT-PCR）结果表明，NnANN 1基因在荷花种子发育和萌发过程中表达量最高，表明NnANN 1基因在植物种子中发挥重要作用。拟南芥中NnANN 1的异位表达导致转基因种子对热胁迫的耐受性增强。此外，与野生型种子相比，异位表达NnANN 1的转基因种子对用于评估种子活力的加速老化处理表现出更好的抗性。此外，转基因种子表现出增强的过氧化物酶活性，伴随着减少脂质过氧化和减少ROS释放水平相比，野生型种子。综上所述，这些结果表明，NnANN 1在种子耐热性和萌发活力中起着重要作用。
Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced annexin, NnANN1, from the embryonic axes of sacred lotus (Nelumbo nucifera Gaertn.) using comparative proteomics approach. Moreover, the expression of NnANN1 increased considerably in response to high-temperature treatment. Quantitative real-time PCR (qRT-PCR) revealed that the transcripts of NnANN1 were detected predominantly during seed development and germination in sacred lotus, implicating a role for NnANN1 in plant seeds. Ectopic expression of NnANN1 in Arabidopsis resulted in enhanced tolerance to heat stress in transgenic seeds. In addition, compared to the wild-type seeds, transgenic seeds ectopically expressing NnANN1 exhibited improved resistance to accelerated aging treatment used for assessing seed vigor. Furthermore, transgenic seeds showed enhanced peroxidase activities, accompanied with reduced lipid peroxidation and reduced ROS release levels compared to the wild-type seeds. Taken together, these results indicate that NnANN1 plays an important role in seed thermotolerance and germination vigor.