Identification of Entomocidal Toxins of Bacillus thuringiensis by High Performance Liquid Chromatography
Identification of Entomocidal Toxins of Bacillus thuringiensis by High Performance Liquid Chromatography
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高效液相色谱法鉴定苏云金芽孢杆菌杀虫毒素
DOI：
10.1099/00221287-129-8-2595
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发表时间：
1983
期刊：
影响因子：
1.5
通讯作者：
Takashi Yamamoto
中科院分区：
文献类型：
--
作者：
Takashi Yamamoto
SUMMARY: Entomocidal proteins produced by certain subspecies of Bacillus thuringiensis were isolated from purified crystals (parasporal bodies) by column chromatography using Sephacryl S-300. The crystals of most subspecies contained only one entomocidal protein (P-1), but some strains of B. thuringiensis which had been classified as subsp. kurstaki, subsp. thuringiensis, subsp. kenyae and subsp. tolworthi appeared to produce an additional protein (P-2) as a minor component of the crystal. The protein (P-2) was serologically similar to the mosquito factor previously discovered in the HD-1 strain of subsp. kurstaki. These entomocidal proteins (P-and P-2) were isolated and digested by trypsin. The peptides resulting from the trypsin digestion were mapped by high performance liquid chromatography (HPLC). HPLC patterns of the toxins, in particular P-1, were reliably reproducible and revealed differences in P-1 toxins between strains even in the same serotype. Analysis of P-2 by HPLC indicated that it is different from P-1 in protein structure.
