Laundry detergent compatibility of the alkaline protease from Bacillus cereus
Laundry detergent compatibility of the alkaline protease from Bacillus cereus
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DOI：
10.1016/j.micres.2004.01.002
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发表时间：
2004-01-01
影响因子：
6.7
通讯作者：
Prakash, M
中科院分区：
文献类型：
--
作者：
Banik, RM;Prakash, M
研究了国际上不同品牌洗衣粉的内源蛋白酶活性。在50 ℃、pH 10.5-11.0范围内，所有测试的洗衣剂的蛋白酶活性均达到最大。在40 ℃下孵育1小时，所测试的洗涤剂中的内源性蛋白酶活性保持高达70%，而在50 ℃下孵育1小时仅发现低于30%的活性。研究了一株嗜碱蜡样芽孢杆菌碱性蛋白酶在工业洗涤剂中的相容性。该菌无细胞摇瓶发酵液在pH10.5、50 ℃时活性最高。来自B的蛋白酶。蜡状芽孢杆菌在50 ℃下与洗涤剂孵育1小时或更长时间后，显示出更高的残留活性（80%以上）。来自B的蛋白酶。发现蜡状蛋白酶与在较高温度下洗涤期间的酶稳定性相比优于存在于测试的商业衣物洗涤剂中的内源性蛋白酶，例如，40- 50 ℃。(C)2004年Elsevier GmbH。All rights reserved.
The endogenous protease activity in various commercially available laundry detergents of international companies was studied. The maximum protease activity was found at 50degreesC in pH range 10.5-11.0 in all the tested laundry detergents. The endogenous protease activity in the tested detergents retained up to 70% on incubation at 40degreesC for 1 h, whereas less than 30% activity was only found on incubation at 50degreesC for 1 h. The alkaline protease from an alkalophilic strain of Bacillus cereus was studied for its compatibitity in commercial detergents. The cell free fermented broth from shake flask culture of the organism showed maximum activity at pH 10.5 and 50degreesC. The protease from B. cereus showed much higher residual activity (more than 80%) on incubation with laundry detergents at 50degreesC for 1 h or longer. The protease enzyme from B. cereus was found to be superior over the endogenous proteases present in the tested commercial laundry detergents in comparison to the enzyme stability during the washing at higher temperature, e.g., 40-50degreesC. (C) 2004 Elsevier GmbH. All rights reserved.