Evidence for the involvement of guanosine 3',5'-cyclic monophosphate in the regulation of gonadotropin release.
Evidence for the involvement of guanosine 3',5'-cyclic monophosphate in the regulation of gonadotropin release.
复制标题
鸟苷 3,5-环单磷酸参与促性腺激素释放调节的证据。
DOI：
10.1210/endo-103-5-1527
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
S. Mccann
中科院分区：
文献类型：
--
作者：
H. Nakano;C. Fawcett;F. Kimura;S. Mccann
向酶促分散的过夜培养的大鼠垂体前叶细胞制剂中加入二丁酰腺苷3 '，5'-环一磷酸（DBcAMP）或二丁酰鸟苷3 '，5'-环一磷酸（DBcGMP; 1- 6 mM），刺激促性腺激素（LH和FSH）从细胞释放到培养液中。DBcGMP刺激促性腺激素释放仅在孵育10分钟后观察到，而DBcAMP引起的刺激在180分钟出现。（2.5 × 10（-9）M）诱导细胞内cAMP含量的小幅、短暂增加（+16%，P <0.05，共孵育5 min后），而相同细胞中cGMP水平在2 h内迅速显著降低。cGMP含量的减少伴随着持续2小时的促性腺激素释放，LH在5分钟后和FSH在10分钟后均可检测到。这些结果强烈提示cGMP可能是LHRH刺激促性腺激素释放过程中的细胞内介质。
Addition of dibutyryl adenosine 3'-5'-cyclic monophosphate (DBcAMP) or dibutyryl guanosine 3',5'-cyclic monophosphate (DBcGMP; 1--6mM) to to enzymatically dispersed, overnight-cultured rat anterior pituitary cell preparations stimulated the release of gonadotropins (LH and FSH) from the cells into the incubation medium. Stimulation of gonadotropin release by DBcGMP was observed after only 10 min of incubation, whereas that caused by DBcAMP appeared at 180 min. Synthetic LHRH (2.5 X 10(-9) M) induced a small, transient increase in intracellular cAMP content (+16%, P less than 0.05, after 5 min coincubation), while levels of cGMP in the same cells were rapidly and markedly low for 2 h. The decrease in cGMP content was accompanied by a discharge of gonadotropins lasting for 2 h, which was detectable after 5 min in the case of LH and after 10 min in the case of FSH. These results strongly suggest that cGMP might be an intracellular mediator in the process of LHRH-stimulated release of gonadotropins.