GANGLIOSIDE G(M3) INHIBITS THE PROLIFERATION OF CULTURED KERATINOCYTES
GANGLIOSIDE G(M3) INHIBITS THE PROLIFERATION OF CULTURED KERATINOCYTES
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DOI：
10.1111/1523-1747.ep12476755
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发表时间：
1993-06-01
影响因子：
6.5
通讯作者：
BREMER, EG
中科院分区：
文献类型：
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作者：
PALLER, AS;ARNSMEIER, SL;BREMER, EG
神经节苷脂G（M3）是角质形成细胞膜的主要神经节苷脂。在其他细胞类型中，已经提出G（M3）可能参与细胞增殖的调节。为了研究G（M3）在角质形成细胞增殖中的作用，将纯化的G（M3）加入到培养的正常包皮、银屑病和鱼鳞病患者皮损皮肤的角质形成细胞和皮肤鳞状细胞癌细胞系中。在10 - 100 μ M的浓度下，补充G（M3）以剂量依赖性方式抑制所有培养的角质形成细胞的生长。银屑病和鱼鳞病患者的角质形成细胞对G（M3）的抑制作用最敏感，汇合的未分化角质形成细胞最不敏感。加入G（M3）后，未观察到分化变化。G（D3）、9-0-乙酰基-G（D3）和G（D1 b）也抑制角质形成细胞增殖。神经节苷脂G（M1）和G（D1 a）和唾液酸的影响不大。向培养的角质形成细胞中加入50 μ M H-3-G（M3）导致细胞G（M3）量的1.7倍。这些数据表明，hematoside（G（M3））和“b "途径神经节苷脂（G（D3），G（D1 b）），由唾液酸转移酶II相对于N-乙酰氨基半乳糖转移酶的优先激活产生，可能参与角质形成细胞生长的控制，但不参与分化。
Ganglioside G(M3) is the predominant ganglioside of keratinocyte membranes. It has been proposed in other cell types that G(M3) may participate in the regulation of cell proliferation. To examine the role of G(M3) in keratinocyte proliferation, purified G(M3) was added to cultured keratinocytes from normal foreskin, from lesional skin of patients with psoriasis and ichthyosis, and to cutaneous squamous carcinoma cell lines. Supplemental G(M3) inhibited the growth of all cultured keratinocytes in a dose-dependent manner at concentrations of 10 - 100 muM. Keratinocytes from patients with psoriasis and ichthyosis were most sensitive to the inhibitory effects of G(M3), and confluent undifferentiated keratinocytes were least sensitive. No change in differentiation was noted after addition of G(M3). G(D3), 9-0-acetyl-G(D3), and G(D1b) also inhibited keratinocyte proliferation. Gangliosides G(M1) and G(D1a) and sialic acid had little effect. Addition of 50 muM H-3-G(M3) to cultured keratinocytes resulted in 1.7 times the amount of cellular G(M3). These data suggest that hematoside (G(M3)) and ''b'' pathway gangliosides (G(D3), G(D1b)), generated by the preferential activation of sialyltransferase II versus N-acetylgalactosaminyltransferase, may be involved in control of keratinocyte growth but not of differentiation.