INHIBITORY EFFECTS OF H-2 ON GROWTH OF CLOSTRIDIUM-CELLOBIOPARUM
INHIBITORY EFFECTS OF H-2 ON GROWTH OF CLOSTRIDIUM-CELLOBIOPARUM
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DOI：
10.1128/aem.31.3.342-348.1976
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发表时间：
1976-01-01
影响因子：
4.4
通讯作者：
CHUNG, KT
中科院分区：
文献类型：
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作者：
CHUNG, KT
H2 抑制产生 H2 的 C. cellobioparum 的生长，但不抑制大肠杆菌或瘤胃拟杆菌的生长。该抑制是可逆的。当通过钯黑或通过向管中放气来去除 H2 时，葡萄糖利用率增加，C. cellobioparum 的光密度和 H2 产量也增加。产甲烷菌去除 H2 有利于 C. cellobioparum 的生长。梭状芽胞杆菌与反刍甲烷杆菌在不同浓度的葡萄糖中一起生长，可达到更高的光密度并产生更多的氢气和更高的活细胞计数。细胞产量也高于纯培养物。在混合培养物中，与纯培养物相比，C. cellobioparum 产生更多的乙酸和更少的乳酸、乙醇和丁酸。讨论了这种代谢转变和氢气利用在产甲烷过程中的重要性。
H2 inhibits the growth of H2-producing C. cellobioparum, but not of Escherichia coli or Bacteroides ruminicola. The inhibition is reversible. When H2 was removed either by palladium black or by gassing out the tube, glucose utilization increased as did optical density and H2 production of C. cellobioparum. Removal of the H2 by methanogenic bacteria favors the growth of C. cellobioparum. Grown with Methanobacterium ruminantium in various concentrations of glucose, the Clostridium reaches a higher optical density and produces more H2 and a higher viable cell count. The cell yield is also higher than in pure culture. In mixed culture, C. cellobioparum produces more acetic acid and less lactic acid, ethanol and butyric acid than in pure culture. The significance of this metabolic shift and H2 utilization in methanogenesis is discussed.