Lower Respiratory Infections Among Hospitalized Children in New Caledonia: A Pilot Study for the Pneumonia Etiology Research for Child Health Project
Lower Respiratory Infections Among Hospitalized Children in New Caledonia: A Pilot Study for the Pneumonia Etiology Research for Child Health Project
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DOI：
10.1093/cid/cir1070
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发表时间：
2012-04-01
影响因子：
11.8
通讯作者：
Levine, Orin S.
中科院分区：
文献类型：
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作者：
Mermond, Sylvain;Zurawski, Virginie;Levine, Orin S.
我们在新喀里多尼亚进行了一项为期一年的前瞻性试点研究，为肺炎儿童健康研究项目做准备。通过痰培养、血培养、尿抗原检测、呼吸道标本PCR和配对血清学等方法对儿童下呼吸道感染的病原体进行鉴定。通过免疫荧光和PCR以及配对血清的血清学检测呼吸道标本中的呼吸道病毒。108例住院患者病原菌检出率为87.9%。病毒占检出的152种病原体的81.6%。呼吸道合胞病毒和鼻病毒是最常见的，分别占已发现病原体的32.2%和24.3%。在采集的99份诱导痰标本中，只有26.3%被确定为质量良好，这可能是所使用的采集方法的结果。
We conducted a prospective pilot study over a 1-year period in New Caledonia in preparation for the Pneumonia Research for Child Health (PERCH) project. The pathogens associated with hospitalized lower respiratory infections in children were identified through the use of culture of induced sputum and blood, urinary antigen detection, polymerase chain reaction (PCR) on respiratory specimens, and serology on paired sera. Respiratory viruses were detected on respiratory specimens by immunofluorescence and PCR, and by serology on paired sera. Pathogens were detected in 87.9% of the 108 hospitalized cases. Viruses represented 81.6% of the 152 pathogens detected. Respiratory syncytial virus and rhinovirus were the most frequent, accounting for 32.2% and 24.3% of the pathogens identified, respectively. Only 26.3% of 99 induced sputum specimens collected were determined to be of good quality, which may be a consequence of the collection method used.