AFLP mapping of soybean maturity gene E4
AFLP mapping of soybean maturity gene E4
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DOI：
10.1093/jhered/esm114
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发表时间：
2008-03-01
影响因子：
3.1
通讯作者：
Takahashi, Ryoji
中科院分区：
文献类型：
--
作者：
Matsumura, Hisakazu;Liu, Baohui;Takahashi, Ryoji
大豆开花和成熟天数受基因E1-E7和J控制。以前的研究表明，E1-E5和E7在不同的强度下对低温诱导的种皮褐变的耐性有不同的影响。E4基因座对早熟耐冷品种的选育具有重要意义，因为隐性等位基因同时控制早熟习性和耐冷性。本研究利用日本地方品种Sakamoowase(E4)和Miharudaizu(E4)杂交的F-8：9家系分离E4，通过AFLP分析获得了E4的精细图谱。AFLP分析共检测到4096对引物，在E4近等基因系之间检测到20个多态标记。连锁作图将16个AFLP标记整合到先前构建的连锁群中E4周围的遗传图谱中，将8个AFLP标记定位在E4与先前发现的最接近标记之间的空白区域。位于E4两侧的两个AFLP标记e48m41-8和e18m38-8分别位于距E4 0.6和5.4 cM的位置。它们是显性的，呈顺式排列，隐性等位基因(E4)控制着光周期不敏感和耐冷性。这些标记可用于开发更精确的标记，用于精细定位和标记辅助选择，以及通过基因组行走方法分离潜在基因。
Days to flowering and maturity are controlled by genes E1-E7 and J in soybean. Previous studies revealed that E1-E5 and E7 influence tolerances to low-temperature-induced seed coat browning in different directions at various intensities. The E4 locus is useful for the development of early maturing cultivars with chilling tolerance because the recessive allele conditions both the early-maturing habit and chilling tolerance. This study was conducted to obtain a fine map of E4 by amplified fragment length polymorphism (AFLP) analysis using a F-8:9 family segregating for E4 that was developed from a cross between photoperiod-insensitive Japanese landraces, Sakamotowase (E4) and Miharudaizu (e4). AFLP analysis using a total of 4096 primer pairs detected 20 polymorphic markers between near-isogenic lines for E4. Linkage mapping incorporated 16 AFLP markers into a previously constructed genetic map around E4 in linkage group I. Eight AFLP markers were localized to unfilled areas between E4 and the closest markers identified previously. Two AFLP markers flanking E4, e48m41-8 and e18m38-8, were mapped at positions 0.6 and 5.4 cM apart from E4, respectively. They were dominant and in cis arrangement with the recessive allele (e4) conditioning the photoperiod insensitivity and chilling tolerance. These markers can be used in developing more precise markers for fine mapping and marker-assisted selection and in isolating the underlying gene via genome walking approaches.