Molecular phylogeny of Cypripedium (Orchidaceae: Cypripedioideae) inferred from multiple nuclear and chloroplast regions
Molecular phylogeny of Cypripedium (Orchidaceae: Cypripedioideae) inferred from multiple nuclear and chloroplast regions
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从多个核和叶绿体区域推断 Cypripedium（兰科：Cypripedioideae）的分子系统发育。
DOI：
10.1016/j.ympev.2011.06.006
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发表时间：
2011-11-01
影响因子：
4.1
通讯作者：
Luo, Yi-bo
中科院分区：
文献类型：
--
作者：
Li, Ji-hong;Liu, Zhong-jian;Luo, Yi-bo
利用nrDNA ITS和五个叶绿体区域（trnH-psbA、atpI-atpH、trnS-trnfM、trnL-F间隔区和trnL内含子）对兰科Cypripedium属的56个分类群进行了分子分析。确认了该属的单系性。我们的数据提供了强有力的支持单系分组的8个亚属部分（亚热带，Obtusipetala，Trigonopedia，Sinopedilum，Bifolia，Flabelinervia，Arietinum，Cypripedium）在早期的分类治疗中定义的，和并系分组的两个部分（和Retinervi）。在Cypripedium属内，第一个分歧的谱系包括两个Mesomaerican物种，随后来自东亚的Cypripedium debile谱系被分裂。我们的研究不支持的概念，两个亚洲物种（Cypripedium subtropicum和Cypripedium singchii）是密切相关的中美洲Cypripedium irapeanum或北美Cypripedium californicum，如以前的解释基于形态学证据。此外，还确认了一对隔行种，即亚洲东部的Cypripedium plectrochilum和北美的Cypripedium arietinum，这是Arietinum组所特有的。此外，在单系组Cypripedium两个以前公认的亚组，Cypripedium和Macrantha，被证明是并系。我们的研究结果表明，这部分分裂成两个组的基础上分布（北美与欧亚大陆），而不是这样以前使用的，形态特征的花的颜色，嘴唇（唇瓣）和侧花瓣的形状。(C)2011 Elsevier Inc. All rights reserved.
A molecular analysis was performed on 56 taxa in the orchid genus Cypripedium using nrDNA ITS and five chloroplast regions (trnH-psbA, atpI-atpH, trnS-trnfM, trnL-F spacer, and the trnL intron). The genus Cypripedium was confirmed as monophyletic. Our data provided strong support for monophyletic grouping of eight infrageneric sections (Subtropica, Obtusipetala, Trigonopedia, Sinopedilum, Bifolia, Flabelinervia, Arietinum, and Cypripedium) defined in earlier taxonomic treatments, and paraphyletic grouping of two sections (Irapeana and Retinervi). Within the genus Cypripedium, the first divergent lineage consisted of two Mesomaerican species, and subsequently the Cypripedium debile lineage from eastern Asia was split. Our study did not support the notion that two Asian species (Cypripedium subtropicum and Cypripedium singchii) were closely related to either Mesoamerican Cypripedium irapeanum or North American Cypripedium californicum, as indicated by previous interpretations based on morphological evidences. In addition, one pair of vicariant species, Cypripedium plectrochilum (eastern Asia) and Cypripedium arietinum (North America), unique to section Arietinum, was confirmed. Furthermore, within the monophyletic section Cypripedium two previously recognized subsections, Cypripedium and Macrantha, were shown to be paraphyletic. Our results suggested that this section split into two groups based on distribution (North America vs. Eurasia) instead of such previously used, morphological traits as flower color, and the shape of the lips (labellum) and lateral petals. (C) 2011 Elsevier Inc. All rights reserved.