The tomato B-type cyclin gene, SlCycB2, plays key roles in reproductive organ development, trichome initiation, terpenoids biosynthesis and Prodenia litura defense
The tomato B-type cyclin gene, SlCycB2, plays key roles in reproductive organ development, trichome initiation, terpenoids biosynthesis and Prodenia litura defense
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番茄 B 型细胞周期蛋白基因 SlCycB2 在生殖器官发育、毛状体起始、萜类生物合成和斜纹夜蛾防御中发挥关键作用
DOI：
10.1016/j.plantsci.2017.05.006
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发表时间：
2017-09-01
期刊：
影响因子：
5.2
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
--
作者：
Gao, Shenghua;Gao, Yanna;Ye, Zhibiao
细胞周期蛋白广泛存在于各种植物中。其中，B型细胞周期蛋白在G2期向M期的转变中起重要作用。然而，很少有报道B型细胞周期蛋白参与生殖器官发育和毛状体的形成。在本研究中，转基因分析表明，SlCycB 2过表达引起异常花，雄蕊不闭合，缩短花柱和异常花粉。此外，几乎所有的非腺毛，以及腺毛消失。相反，抑制SlCycB 2可以促进III型和V型毛状体的形成。次生代谢产物的检测表明，SICycB 2-OE植株中单萜和倍半萜的产量显著降低，从而导致对斜纹夜蛾的防御能力降低。转录组分析表明，差异表达基因主要参与萜烯类、角质类、蜡类和软木类物质的生物合成。此外，我们还鉴定了SlCycB 2，SlCycB 3，NtCycB 2，AtCycB 2的几个同源物，它们在毛状体形成中具有相似的调节功能。这些结果表明SlCycB 2在番茄生殖器官发育、多细胞毛状体发生、次生代谢产物合成和斜纹夜蛾防御中起着关键作用。其同源物在多细胞毛状体形成中的相似作用表明茄科物种可能具有共同的调控途径。
Cyclins exist extensively in various plant species. Among them, B-type cyclins play important roles in the transition of G2-to-M. However, few B-type cyclins have been reported to participate in reproductive organ development and trichome formation. In this study, transgene analysis showed that SlCycB2 overexpression caused abnormal flower with the unclosed stamen, shortened style and aberrant pollen. In addition, nearly all non-glandular trichomes, as well as the glandular ones were disappeared. On the contrary, suppression of SlCycB2 could promote type III and type V trichomes formation. Detection of secondary metabolites indicated that the production of monoterpene and sesquiterpene were significantly decreased in SICycB2-OE plants, which thus resulted in the reduction of the defense against Prodenia litura. Transcriptome profile demonstrated that the differentially expressed genes mainly participate in the biosynthesis of terpenes, cutin, suberine and wax. Furthermore, we identified several homologs of SlCycB2, SlCycB3, NtCycB2, AtCycB2, which have similar regulatory functions in trichome formation. These results indicate that SlCycB2 plays a critical role in reproductive organ development, multicellular trichome initiation, secondary metabolite biosynthesis and Prodenia litura defense in tomato. The similar roles of its homologs in multicellular trichome formation suggest that Solanaceous species may share common regulatory pathway.