PIOX, a New Pathogen-Induced Oxygenase with Homology to Animal Cyclooxygenase
PIOX, a New Pathogen-Induced Oxygenase with Homology to Animal Cyclooxygenase
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PIOX，一种与动物环加氧酶同源的新型病原体诱导加氧酶
DOI：
10.1105/tpc.10.9.1523
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发表时间：
1998
期刊：
影响因子：
11.6
通讯作者：
C. Castresana
中科院分区：
文献类型：
--
作者：
A. Sanz;J. Moreno;C. Castresana
检查了 harpin HrpN 蛋白诱导子在烟草叶中诱导的基因表达变化，并鉴定了一种新的 cDNA，piox（病原体诱导的加氧酶），与编码环氧合酶或前列腺素内过氧化物合酶 (PGHS) 的基因具有同源性。除了确定的氨基酸同一性外，还预测 piox 编码的蛋白质具有与绵羊 PGHS-1 相似的结构核心。此外，蛋白质功能的研究表明，PIOX重组蛋白至少具有PGHS的两种酶活性之一，即催化多不饱和脂肪酸的氧化。蛋白质引发剂处理或接种细菌后，piox 转录物积累。接种不相容和相容细菌的组织中都会诱导 piox 的表达，但两种类型的相互作用的 RNA 和蛋白质积累有所不同。我们发现，piox 的表达会响应介导植物对病原体感染反应的各种细胞信号而迅速诱导，并且 piox 表达的激活很可能与所检查的细胞死亡过程中发生的氧化爆发有关。环氧合酶催化前列腺素和血栓素形成的第一个关键步骤，前列腺素和血栓素是脂质衍生的信号分子，介导许多细胞过程，包括脊椎动物的免疫反应。烟草 PIOX 的发现表明，植物和动物系统基于脂质的反应之间将发现比迄今为止预期更多的相似性。
Changes in gene expression induced in tobacco leaves by the harpin HrpN protein elicitor were examined, and a new cDNA, piox (for pathogen-induced oxygenase), with homology to genes encoding cyclooxygenase or prostaglandin endoperoxide synthase (PGHS), was identified. In addition to the amino acid identity determined, the protein encoded by piox is predicted to have a structural core similar to that of ovine PGHS-1. Moreover, studies of protein functionality demonstrate that the PIOX recombinant protein possesses at least one of the two enzymatic activities of PGHSs, that of catalyzing the oxygenation of polyunsaturated fatty acids. piox transcripts accumulated after protein elicitor treatment or inoculation with bacteria. Expression of piox was induced in tissues responding to inoculation with both incompatible and compatible bacteria, but RNA and protein accumulation differed for both types of interactions. We show that expression of piox is rapidly induced in response to various cellular signals mediating plant responses to pathogen infection and that activation of piox expression is most likely related to the oxidative burst that takes place during the cell death processes examined. Cyclooxygenase catalyzes the first committed step in the formation of prostaglandins and thromboxanes, which are lipid-derived signal molecules that mediate many cellular processes, including the immune response in vertebrates. The finding of tobacco PIOX suggests that more similarities than hitherto expected will be found between the lipid-based responses for plant and animal systems.
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DOI：
10.1073/pnas.85.5.1412
发表时间：
1988-03-01
影响因子：
11.1
作者：
DEWITT, DL;SMITH, WL
通讯作者：
SMITH, WL
DOI：
10.1073/pnas.89.11.4888
发表时间：
1992-06-01
影响因子：
11.1
作者：
OBANION, MK;WINN, VD;YOUNG, DA
通讯作者：
YOUNG, DA
DOI：
10.1073/pnas.93.22.12053
发表时间：
1996-10-29
影响因子：
11.1
作者：
Bergey, DR;Howe, GA;Ryan, CA
通讯作者：
Ryan, CA
影响因子：
3.9
作者：
FENG, L;SUN, WQ;HWANG, D
通讯作者：
HWANG, D