Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation
Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation
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拟南芥紫色酸性磷酸酶 AtPAP10、AtPAP12 和 AtPAP26 的比较遗传分析为它们在植物适应磷酸盐匮乏中的作用提供了新的见解
DOI：
10.1111/jipb.12184
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发表时间：
2014-03-01
影响因子：
11.4
通讯作者：
Liu, Dong
中科院分区：
文献类型：
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作者：
Wang, Liangsheng;Lu, Shan;Liu, Dong
酸性磷酸酶（APases）的诱导和分泌被认为是植物在缺磷条件下生存和生长的一种适应机制。在拟南芥中，有29个紫色酸性磷酸酶（AtPAP）基因。为了系统地研究不同AtPAP的作用，我们首先鉴定了所有29个AtPAP基因的敲除或敲低T-DNA系。利用这些atpap突变体结合凝胶内和定量APase酶分析，我们证明了AtPAP 12和AtPAP 26是拟南芥中两个主要的细胞内和分泌型APase，而AtPAP 10主要是分泌型APase。在Pi缺乏（P-）培养基或补充有有机磷酸ADP和果糖-6-磷酸（Fru-6-P）的P-培养基上，atpap 10的生长显著降低，而atpap 12的生长仅中度降低，atpap 26的生长几乎与野生型（WT）的生长相等。然而，AtPAP 12或AtPAP 26基因的过表达导致植物在补充有ADP或Fru-6-P的P-或P-培养基上生长得更好。有趣的是，WT和过表达系的Pi水平基本相同，尽管这两种类型的植物具有显著不同的生长表型。这些结果表明，APases可能有其他的作用，除了加强内部的磷回收或释放磷从外部有机磷植物吸收。
Induction and secretion of acid phosphatases (APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate (Pi) deprivation. In Arabidopsis, there are 29 purple acid phosphatase (AtPAP) genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock-down T-DNA lines for all 29 AtPAP genes. Using these atpap mutants combined with in-gel and quantitative APase enzyme assays, we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in Arabidopsis while AtPAP10 is mainly a secreted APase. On Pi-deficient (P-) medium or P- medium supplemented with the organophosphates ADP and fructose-6-phosphate (Fru-6-P), growth of atpap10 was significantly reduced whereas growth of atpap12 was only moderately reduced, and growth of atpap26 was nearly equal to that of the wild type (WT). Overexpression of the AtPAP12 or AtPAP26 gene, however, caused plants to grow better on P- or P- medium supplemented with ADP or Fru-6-P. Interestingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake.