Expression and function of FGF-4 in peri-implantation development in mouse embryos.
Expression and function of FGF-4 in peri-implantation development in mouse embryos.
复制标题
DOI：
--
复制
发表时间：
1994-08
期刊：
影响因子：
4.6
通讯作者：
D. Rappolee;C. Basilico;Yogesh Patel;Z. Werb
中科院分区：
文献类型：
--
作者：
D. Rappolee;C. Basilico;Yogesh Patel;Z. Werb
哺乳动物胚胎发生中最早的事件之一是内细胞团（ICM）的形成和随后的原始内胚层分层。我们发现成纤维细胞生长因子（FGF）-4（而非 FGF-3）的 mRNA 在植入前小鼠囊胚中表达，并且 FGF-4 多肽存在于 ICM 细胞中。 ICM样胚胎癌细胞和胚胎干细胞也表达FGF-4。相反，内胚层谱系中的分化胚胎癌细胞表达 FGF-3，但不表达 FGF-4 mRNA。尽管小鼠胚胎从 1 细胞阶段开始表达 FGF-4 mRNA，但在重组 FGF-4 存在的情况下从 2 细胞培养到囊胚阶段的胚胎没有促有丝分裂反应。然而，当通过免疫手术分离的 ICM 与 FGF-4 一起培养时，生长到盖玻片上的形态不同、分化的壁内胚层细胞的数量增加，而未分化的 ICM 细胞的数量没有增加。用 FGF-4 培养的 ICM 生长物增加了 92 x 10(3) M(r) 明胶酶和组织纤溶酶原激活剂的分泌，这是迁移细胞的标志。 FGF-4 受体（FGFR-3 和 FGFR-4）在小鼠囊胚的所有细胞中表达。这些发现表明，未分化的 ICM 细胞产生的 FGF-4 在胚胎发生的围植入期发挥作用，影响源自其的内胚层细胞的产生和行为。
One of the earliest events in mammalian embryogenesis is the formation of the inner cell mass (ICM) and the subsequent delamination of primitive endoderm. We have found that mRNA for fibroblast growth factor (FGF)-4, but not FGF-3, is expressed in preimplantation mouse blastocysts and that the FGF-4 polypeptide is present in ICM cells. ICM-like embryonal carcinoma cells and embryonic stem cells also express FGF-4. Conversely, differentiated embryonal carcinoma cells in the endoderm lineage express FGF-3, but not FGF-4 mRNA. Although mouse embryos expressed FGF-4 mRNA from the 1-cell stage, embryos cultured from the 2-cell through the blastocyst stage in the presence of recombinant FGF-4 did not respond mitogenically. However, when ICMs that were isolated by immunosurgery were cultured with FGF-4, the number of morphologically distinct, differentiated parietal endoderm cells growing out onto the coverslip increased, without an increase in the number of undifferentiated ICM cells. ICM outgrowths cultured with FGF-4 increased their secretion of 92 x 10(3) M(r) gelatinase and tissue plasminogen activator, a hallmark of migrating cells. Receptors for FGF-4 (FGFR-3 and FGFR-4) are expressed in all cells of the mouse blastocyst. These findings indicate that FGF-4 produced by undifferentiated ICM cells acts in the peri-implantation period of embryogenesis to influence the production and behavior of endoderm cells derived from them.