Comparison of the effects of sucrose and fructose on insulin action and glucose tolerance
Comparison of the effects of sucrose and fructose on insulin action and glucose tolerance
复制标题
DOI：
10.1152/ajpregu.2000.279.4.r1334
复制
发表时间：
2000-10-01
影响因子：
2.8
通讯作者：
Pagliassotti, MJ
中科院分区：
文献类型：
--
作者：
Thresher, JS;Podolin, DA;Pagliassotti, MJ
本研究的目的是确定果糖是否是蔗糖诱导的胰岛素抵抗和葡萄糖耐受不良的营养介质。为此，雄性大鼠被喂食纯淀粉饮食（占总热量的68%）2周的基线期。在此之后，大鼠要么继续吃淀粉（ST）饮食，要么换成蔗糖（SU，占总热量的68%）、果糖/葡萄糖（F/G，占总热量的34/34%）或果糖/淀粉（F/ST，占总热量的34/34%）饮食，持续5周。然后，大鼠进行静脉葡萄糖耐量试验（n = 10/日粮）或正血糖、高胰岛素钳夹（n = 8或9/日粮）。SU、F/G和F/ST的增量葡萄糖和胰岛素曲线下面积分别平均比ST大61和29%，但彼此之间没有显著差异。钳夹期间，维持血糖正常所需的葡萄糖输注率(mg.kg（-1).min(-1)）在SU、F/G和F/ST（分别为13.4 +/- 0.9、9.5 +/- 1.7和11.3 +/- 1.3）与ST（22.8 +/- 1.1）相比显著降低（P < 0.05）。胰岛素对葡萄糖外观(mg.kg（-1).min(-1)）的抑制作用在SU、F/G和F/ST组（分别为5.6 +/- 0.5、2.2 +/- 1.2和6.6 +/- 0.7）显著低于ST组（9.6 +/- 0.4）（P < 0.05）。胰岛素刺激的葡萄糖消失量(mg.kg（-1).min(-1)）在SU、F/G和F/ST组（分别为17.9 +/- 0.6、16.2 +/- 1.3和15.3 +/- 1.8）显著低于ST组（24.7 +/- 1.2）（P < 0.05）。这些数据表明果糖是蔗糖诱导的胰岛素抵抗和葡萄糖耐受不良的主要营养介质。
The purpose of the present study was to determine whether fructose is the nutrient mediator of sucrose-induced insulin resistance and glucose intolerance. Toward this end, male rats were fed a purified starch diet (68% of total calories) for a 2-wk baseline period. After this, rats either remained on the starch (ST) diet or were switched to a sucrose (SU, 68% of total calories), fructose/glucose (F/G, 34/34% of total calories), or fructose/starch (F/ST, 34/34% of total calories) diet for 5 wk. Rats then underwent either an intravenous glucose tolerance test (n = 10/diet) or a euglycemic, hyperinsulinemic clamp (n = 8 or 9/diet). Incremental glucose and insulin areas under the curve in SU, F/G, and F/ST were on average 61 and 29% greater than ST, respectively, but not significantly different from one another. During clamps, glucose infusion rates (mg.kg(-1).min(-1)) required to maintain euglycemia were significantly lower (P < 0.05) in SU, F/G, and F/ST (13.4 +/- 0.9, 9.5 +/- 1.7, 11.3 +/- 1.3, respectively) compared with ST (22.8 +/- 1.1). Insulin suppression of glucose appearance (mg.kg(-1).min(-1)) was significantly lower (P < 0.05) in SU, F/G, and F/ST (5.6 +/- 0.5, 2.2 +/- 1.2, and 6.6 +/- 0.7, respectively) compared with ST (9.6 +/- 0.4). Insulin-stimulated glucose disappearance (mg.kg(-1).min(-1)) was significantly lower (P < 0.05) in SU, F/G, and F/ST (17.9 +/- 0.6, 16.2 +/- 1.3, 15.3 +/- 1.8, respectively) compared with ST (24.7 +/- 1.2). These data suggest that fructose is the primary nutrient mediator of sucrose-induced insulin resistance and glucose intolerance.