Comparison of genotyping of Helicobacter pylori cagA and vacA virulence genes from gastric biopsies and stool specimens.
Comparison of genotyping of Helicobacter pylori cagA and vacA virulence genes from gastric biopsies and stool specimens.
复制标题
胃活检和粪便标本中幽门螺杆菌 cagA 和 vacA 毒力基因的基因分型比较。
DOI：
10.1111/j.1523-5378.2003.00181.x
复制
发表时间：
2003
期刊：
影响因子：
4.4
通讯作者：
Schneider,BarbaraG
中科院分区：
文献类型：
--
作者：
Sicinschi,LiviuA;Correa,Pelayo;Schneider,BarbaraG
Background.We compared results of genotyping ofHelicobacter pylori cagAandvacAvirulence genes in DNA from gastric biopsies, both paraffin‐embedded and frozen, and from stool samples, in order to evaluate the comparative sensitivity of the stool assay.Methods.Genomic DNA from paraffin‐embedded biopsies, unfixed frozen biopsies, and stool samples of the same 20 patients was amplified for thecagAgene, an empty site (which provides a positive signal forcagAnegative strains) and for the s and m alleles of thevacAgene. Composite genotypes were determined by combining data from analysis of all three materials.Results.Analysis of none of the materials taken singly showed all of the genotypes revealed by all three materials taken together, probably because of sampling error. Analysis of paraffin biopsies revealed 83.5%, that of frozen biopsies revealed 74.7% and that of stools revealed 75.9% of the genotypes. There was no significant difference in the percentage of theH. pylorigenotypes identified from the three materials. Analysis of combinations of frozen biopsies and stools revealed 89.9% of the composite genotypes, and that of paraffin biopsies and stools revealed 96.2% of the composite genotypes. Evidence of multiple genotypes was found in 10 of 20 (50%) of the cases.Conclusions.Any one of the investigated biological materials can be used for detection ofcagAandvacAgenes, but no single assay provided a complete genotype. The use of a combination of two materials may generate a more accurate representation ofH. pylorigenotypes in each individual.
