DEVELOPMENTAL FUNCTION OF ELF-1 - AN ESSENTIAL TRANSCRIPTION FACTOR DURING EMBRYOGENESIS IN DROSOPHILA
DEVELOPMENTAL FUNCTION OF ELF-1 - AN ESSENTIAL TRANSCRIPTION FACTOR DURING EMBRYOGENESIS IN DROSOPHILA
复制标题
DOI：
10.1101/gad.5.9.1672
复制
发表时间：
1991-09-01
影响因子：
10.5
通讯作者：
KAFATOS, FC
中科院分区：
文献类型：
--
作者：
BRAY, SJ;KAFATOS, FC
果蝇转录因子Elf-1与多巴脱羧酶基因（Ddc）神经元表达所必需的顺式作用元件结合。 Elf-1还在体外刺激Ddc和Ultrabithorax启动子的转录。 为了研究这种因子在体内的功能，我们筛选了突变，并确定了Elf-1基因为grainyhead（grh），以前已知的胚胎致死位点。 Elf-1/grh突变导致晚期胚胎死亡，伴随着角质层和头部骨骼的多重缺陷。 使用Ddc-lacZ基因融合，我们表明，这些突变影响Ddc在胚胎中的表达。 然而，令人惊讶的是，表皮表达被破坏，而神经元表达保持不受影响。 突变表型的分析表明，Elf-1协调调节参与表皮结构分化的多个基因。 这些结果突出了遗传分析在研究基因表达的发育调控所需的蛋白质中的重要性。
The Drosophila transcription factor Elf-1 binds to a cis-acting element that is essential for neuronal expression of the Dopa decarboxylase gene (Ddc). Elf-1 also stimulates transcription from the Ddc and Ultrabithorax promoters in vitro. To investigate the function of this factor in vivo we have screened for mutations and identified the Elf-1 gene as grainyhead (grh), a previously known embryonic lethal locus. Elf-1/grh mutations cause late embryonic lethality accompanied by multiple defects in the cuticle and head skeleton. Using Ddc-lacZ gene fusions, we show that these mutations affect Ddc expression in the embryo. Surprisingly, however, epidermal expression is disrupted, whereas neuronal expression remains unaffected. Analysis of the mutant phenotype indicates that Elf-1 coordinately regulates multiple genes involved in the differentiation of epidermal structures. The results highlight the importance of genetic analysis in the study of proteins required for developmental regulation of gene expression.