Detection and developmental changes of the 1,25-(OH)2-D3 receptor concentration in mouse skin and intestine.
Detection and developmental changes of the 1,25-(OH)2-D3 receptor concentration in mouse skin and intestine.
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小鼠皮肤和肠道1,25-(OH)2-D3受体浓度的检测和发育变化。
DOI：
10.1111/1523-1747.ep12272358
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Holick,MF
中科院分区：
文献类型：
--
作者：
Horiuchi,N;Clemens,TL;Schiller,AL;Holick,MF
我们研究了胎儿、新生儿和成年小鼠皮肤中的[3H] 1,25 -二羟基维生素d3特异性结合活性，以确定(a)皮肤在发育的哪个阶段发展出对这种激素作出反应的能力，以及(b)激素结合活性在发育和成熟过程中是否发生变化。在胎龄17天的小鼠幼崽的皮肤和肠道中检测到一种与小鸡肠道1,25-二羟基维生素d3受体性质相似的大分子。1,25-二羟基维生素d3特异性结合活性在3.5-3.7S的线性蔗糖梯度上沉淀，在0.22 M KCI下从DNA纤维素中洗脱。在胎儿生命的早期阶段（12-14天），从全鼠胎儿制备的细胞质中检测到受体样活性。1,25-二羟基维生素d3特异性结合活性在整个发育过程中使用染色质结合测定在皮肤和肠道中定量。饱和结合数据的Scatchard分析显示，小鼠出生后皮肤中结合活性浓度迅速升高，在10-19日龄时达到最大值。相比之下，在胎儿和新生儿全肠中检测到的结合活性一直很低，直到小鼠断奶。结合活性的亲和力（Kd）在所有年龄的皮肤和肠道中相似。由此可见，1,25-二羟基维生素d3特异性结合活性在小鼠出生之前就存在于皮肤和肠道中，并在发育的不同阶段在这两个组织中有所增加。
We investigated [3H] 1, 25-dihydroxyvitamin D3-specific binding activity in fetal, neonatal, and adult mouse skin to determine (a) during which stage in development the skin develops the capacity to respond to this hormone and (b) whether the hormone binding activity changed during development and maturation.A macromolecule with properties similar to those of the chick intestinal 1,25-dihydroxyvitamin D3receptor was detected in the skin and intestine of mouse pups at 17 days of fetal life. 1,25-Dihydroxyvitamin D3-specific binding activity from both tissues sedimented on linear sucrose gradients at 3.5–3.7S and eluted from DNA cellulose at 0.22 M KCI. At earlier stages of fetal life (12–14 days) receptor-like activity was detected in cytosols prepared from whole-mouse fetuses.1,25-Dihydroxyvitamin D3-specific binding activity was quantitated in the skin and intestine throughout development using a chromatin binding assay. Scatchard analysis of saturation binding data showed that the concentration of binding activity in skin increased rapidly after birth and reached a maximum when the mice were 10–19 days old. By contrast, the binding activity that was detected in the fetal and neonatal whole intestine remained low until the mice were weaned. The affinity (Kd) of the binding activity was similar in skin and intestine at all ages studied. It is concluded that 1,25-dihydroxyvitamin D3-specific binding activity appears in both skin and intestine of the mouse prior to birth and increases in these two tissues during different stages in development.