HIPPOCAMPAL PLASTICITY INDUCED BY PRIMED BURST, BUT NOT LONG-TERM POTENTIATION, STIMULATION IS IMPAIRED IN AREA CA1 OF AGED FISCHER-344 RATS
HIPPOCAMPAL PLASTICITY INDUCED BY PRIMED BURST, BUT NOT LONG-TERM POTENTIATION, STIMULATION IS IMPAIRED IN AREA CA1 OF AGED FISCHER-344 RATS
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DOI：
10.1002/hipo.450030106
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发表时间：
1993-01-01
期刊：
影响因子：
3.5
通讯作者：
ROSE, GM
中科院分区：
文献类型：
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作者：
MOORE, CI;BROWNING, MD;ROSE, GM
两种类型的电刺激的效果，旨在诱导持久的可塑性的谢弗/连合输入CA 1锥体神经元进行了研究，在体外海马切片从年轻（3-6个月）和老年（24-27个月）Fischer 344大鼠。第一种刺激范例，预激脉冲串（PB）刺激，由总共五个生理模式的刺激：一个单一的预激脉冲，随后170毫秒后，由四个脉冲在200 Hz的脉冲串。第二种刺激范例，长时程增强（LTP）刺激，由200 Hz/1秒的序列（总共200个刺激）组成。引发爆发和LTP刺激同样有效地诱导从年轻大鼠切片记录的群体尖峰的持久增加。然而，增强群体尖峰幅度产生的PB，但不是LTP，刺激是显着减少从老年大鼠切片。这些结果表明，海马显示持久突触可塑性的能力并不随年龄而改变，但参与可塑性诱导机制变得更加困难。此外，这些数据表明，诱导持久的突触可塑性的生理模式的范例可以更准确地评估海马记忆编码机制的功能状态比传统的LTP刺激。
The effect of two types of electrical stimulation designed to induce long-lasting plasticity of the Schaffer/commissural inputs to CA1 pyramidal neurons was investigated using in vitro hippocampal slices made from young (3-6 month) and old (24-27 month) Fischer 344 rats. The first stimulation paradigm, primed burst (PB) stimulation, consisted of a total of five physiologically patterned stimuli: a single priming pulse followed 170 ms later by a burst of four pulses at 200 Hz. The second stimulation paradigm, long-term potentiation (LTP) stimulation, consisted of a 200 Hz/1 second train (a total of 200 stimuli). Primed burst and LTP stimulation were equally effective at inducing a lasting increase in the population spike recorded from slices made from young rats. However, the enhancement of population spike amplitude produced by PB, but not LTP, stimulation was significantly less in slices made from old rats. These results suggest that the capacity of the hippocampus to demonstrate long-lasting synaptic plasticity is not altered with age, but that engaging plasticity-inducing mechanisms becomes more difficult. Furthermore, these data suggest that physiologically patterned paradigms for inducing long-lasting synaptic plasticity may more accurately assess the functional status of hippocampal memory encoding mechanisms than does conventional LTP stimulation.