Broadening of DNA replication origin usage during metazoan cell differentiation
Broadening of DNA replication origin usage during metazoan cell differentiation
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DOI：
10.1038/sj.embor.7400736
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发表时间：
2006-08-01
期刊：
影响因子：
7.7
通讯作者：
Prioleau, Marie-Noelle
中科院分区：
文献类型：
--
作者：
Dazy, Sebastien;Gandrillon, Olivier;Prioleau, Marie-Noelle
我们研究了在原代红系祖细胞分化成红细胞的过程中，鸡β-珠蛋白基因座的复制是否发生变化。在未分化的祖细胞中，观察到四个主要起始位点和一个复制叉暂停区（RFP）。诱导分化后48小时，主要位点保持不变，即使在活化的β（A）-珠蛋白基因中，一些次要位点也增强了，出现了3个新的位点，RFP消失。其中一个激活的起源显示组蛋白H3 K9 K14双乙酰化增加，但其他的没有。这些结果表明，未转化的后生动物细胞分化过程中的DNA复制起点的使用范围扩大，并表明组蛋白H3双乙酰化，其他组蛋白修饰，迄今报道和转录在这个系统中的起源选择不是至关重要的决定因素。
We have examined whether replication of the chicken beta-globin locus changes during differentiation of primary erythroid progenitors into erythrocytes. In undifferentiated progenitors, four principal initiation sites and a replication fork pausing region (RFP) were observed. Forty-eight hours after induction of differentiation, the principal sites were maintained, even in the activated beta(A)-globin gene, some minor sites were enhanced, three new sites appeared and the RFP disappeared. One of the activated origins showed increased histone H3 K9K14 diacetylation, but the others did not. These results demonstrate a broadening of DNA replication origin usage during differentiation of untransformed metazoan cells and indicate that histone H3 diacetylation, other histone modifications so far reported and transcription are not crucial determinants of origin selection in this system.