Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections
Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections
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DOI：
10.1128/jcm.01866-06
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发表时间：
2007-01-01
影响因子：
9.4
通讯作者：
Struelens, M. J.
中科院分区：
文献类型：
--
作者：
Hallin, M.;Deplano, A.;Struelens, M. J.
自1992年以来，比利时葡萄球菌参考实验室已将基因组宏限制性片段的脉冲场凝胶电泳（PFGE）用于耐甲氧西林金黄色葡萄球菌的国家医院调查。蛋白A基因多态性X区域的测序（SPA分型）在速度、易于解释和可出口性方面提供了优于PFGE的显著优势。为了验证其在国家监测中的潜在用途，我们评估了SPA分型与基于217 S收集的PFGE分型相比的稳健性。金黄色葡萄球菌菌株代表比利时S.在过去的13年里，金葡菌流行病学。SPA分型和PFGE分型的判别力分别为0.98和0.96，两种方法的分型符合率为95.9%。两种方法与多位点序列分型（MLST）的符合率均为95.5%。然而，我们观察到偶尔的“违规”MLST克隆复合物分配SPA分型。我们的研究结果表明，PFGE和SPA分型是长期的，全国流行病学监测研究的可靠方法。我们建议，SPA分型，这是一个单一的位点为基础的方法，最好是与其他标志物，如葡萄球菌盒式染色体MEC分型或耐药或毒力基因检测相结合。
Pulsed-field gel electrophoresis (PFGE) of genomic macrorestriction fragments has been used by the Belgian Reference Laboratory for Staphylococci for national hospital surveys of methicillin-resistant Staphylococcus aureus since 1992. The sequencing of the polymorphic X region of the protein A gene (spa typing) offers significant advantages over PFGE in terms of speed, ease of interpretation, and exportability. To validate its potential use for national surveillance, we evaluated the robustness of spa typing compared with that of PFGE based on a collection of 217 S. aureus strains representative of the Belgian S. aureus epidemiology during the last 13 years. spa typing and PFGE both showed high discriminatory power (discriminatory indexes of 0.98 and 0.96, respectively) and achieved high concordance (95.9%) in type classification. Both methods also showed good concordance with multilocus sequence typing (MLST) (95.5%). However, we observed occasional "violations" of MLST clonal complex assignment by spa typing. Our results suggest that both PFGE and spa typing are reliable methods for long-term, nationwide epidemiological surveillance studies. We suggest that spa typing, which is a single-locus-based method, should preferably be used in combination with additional markers, such as staphylococcal cassette chromosome mec typing or resistance or virulence gene detection.