Generation and Analysis of ESTs from the Grass Carp, Ctenopharyngodon idellus
Generation and Analysis of ESTs from the Grass Carp, Ctenopharyngodon idellus
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DOI：
10.1080/10495398.2010.505843
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发表时间：
2010-10
影响因子：
3.7
通讯作者：
Bing Xu;Shaolin Wang;Yan Jiang;Linlin Yang;Ping Li;C. Xie;Jubin Xing;Zhonghe Ke;Jiale Li;Junwei Gai;Guimei Yang;B. Bao;Zhanjiang Liu
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文献类型：
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作者：
Bing Xu;Shaolin Wang;Yan Jiang;Linlin Yang;Ping Li;C. Xie;Jubin Xing;Zhonghe Ke;Jiale Li;Junwei Gai;Guimei Yang;B. Bao;Zhanjiang Liu
草鱼，Ctenopharyngodon idellus （Valenciennes, 1844）是世界上广泛养殖的重要经济物种，但其基因组研究资源严重缺乏。本研究的目的是构建标准化的cDNA文库，用于高效的EST分析，从这些文库中生成EST，并鉴定EST相关的分子标记，如微卫星和单核苷酸多态性（snp），用于该物种的遗传分析。总共产生了6,269条ESTs，代表4,815个独特序列，从中鉴定了105个推定的微卫星和5,228个snp。这些基因组资源为今后对该物种进行遗传和功能分析提供了物质基础。
Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.