Development of a diagnostic assay for differentiation between genetic groups in clades I, II, III and IV of Puccinia graminis f. sp. tritici.
Development of a diagnostic assay for differentiation between genetic groups in clades I, II, III and IV of Puccinia graminis f. sp. tritici.
复制标题
开发用于区分禾本科柄锈菌 f 分支 I、II、III 和 IV 遗传群体的诊断测定法。
作者：
L. Szabo;P. O. Olivera Firpo;R. Wanyera;B. Visser;Yue Jin
小麦秆锈病是由禾柄锈菌新变种引起的一种严重病害。白藓具有显著遗传性和广泛地理分布（非洲、中亚和欧洲）的变异体包括Ug99种族组、种族TTRTF和种族TKTTF。遗传分析已经将代表这些关键的新毒性小种的分离株分成12个遗传组，构成进化枝I至IV。针对这12个遗传组的分子诊断检测方法的开发将成为全球监测工作的重要组成部分。一个单核苷酸多态性数据库挖掘候选标记，将区分这12个遗传组。筛选了35个候选标记，并对一组94株代表广泛的基因型和种族表型的17个标记的核心集进行了测试。该核心标记集在鉴定进化枝I至IV中的52个分离株的12个遗传组中是100%准确的，并且在非靶分离株中没有观察到假阳性。该检测试剂盒具有内置冗余，因此微小的遗传变化或基因分型调用错误不会影响结果的准确性。该测定法还有效鉴定来自德国和格鲁吉亚的进化枝V中的遗传组，北美进化枝VI中的三个主要亚组，以及由在北美和南美发现的小种TTTTF组成的进化枝VII。该检测方法为活体和非活体样品的快速诊断提供了一种工具，用于检测禾谷缢管蚜的这些重要新小种或小类群。白藓
Wheat stem rust has re-emerged as a serious disease caused by new variants of Puccinia graminis f. sp. tritici. Variants with significant virulences and broad geographical distribution (Africa, Central Asia, and Europe) include the Ug99 race group, race TTRTF, and TKTTF race group. Genetic analysis has placed isolates representing these critical new virulent races into 12 genetic groups that make up clades I to IV. Development of molecular diagnostic assay for these 12 genetic groups will be an important component of global surveillance efforts. A single-nucleotide polymorphism database was mined for candidate markers that would differentiate between these 12 genetic groups. Thirty-five candidate markers were screened and a core set of 17 markers were tested against a set of 94 isolates representing a broad range of genotypes and race phenotypes. This core set of markers were 100% accurate in identifying the 12 genetic groups for 52 isolates in clades I to IV and no false positives were observed with non-target isolates. The assay has built-in redundancy so that minor genetic changes, or errors in genotyping calling will not affect the accuracy of the results. This assay is also effective in identifying the genetic groups in clade V from Germany and Georgia, the three main subgroups in North American clade VI, and the clade VII consisting of race TTTTF found in North and South America. This assay provides a rapid diagnostic tool for both living and non-living samples for detecting these critical new race or race groups of P. graminis f. sp. tritici.