Sensitive and Versatile Detection of the Fouling Process and Fouling Propensity of Proteins on Polyvinylidene Fluoride Membranes via Surface-Enhanced Raman Spectroscopy
Sensitive and Versatile Detection of the Fouling Process and Fouling Propensity of Proteins on Polyvinylidene Fluoride Membranes via Surface-Enhanced Raman Spectroscopy
复制标题
通过表面增强拉曼光谱灵敏且多功能地检测聚偏二氟乙烯膜上蛋白质的污染过程和污染倾向
DOI：
10.1021/ac102891g
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发表时间：
2011-03-01
影响因子：
7.4
通讯作者：
Zhang, Kai-Song
中科院分区：
文献类型：
--
作者：
Cui, Li;Yao, Meng;Zhang, Kai-Song
膜污染是膜基技术的主要缺点，因为它会导致通量严重下降，需要清洗或更换污染的膜。一种能够早期诊断、过程监测和评估不同污染物在污染过程中所起作用的技术对于污染控制至关重要。我们开发了表面增强拉曼光谱（SERS）作为一种新的多功能工具来研究蛋白质在PVDF（聚偏氟乙烯）膜上的污垢过程以及三种不同蛋白质的污垢倾向。我们优化了sers活性Ag溶胶的聚集水平和体积，以及光谱采集方法与统计分析方法相结合，以确保高检测灵敏度、信号均匀性和稳定性。然后，我们使用SEAS对污染过程进行早期诊断，并确定膜孔何时会被堵塞。通过银染色和拉曼成像的结合来显示污染区域。通过比较不同蛋白质在玻片上和膜过滤后的相对SERS波段强度，研究了不同蛋白质的污垢倾向。与基于荧光的技术相比，窄且分辨率高的拉曼带，特别是使用相同的激发线和激光功率，使SEAS能够以非常简单的方式比较结垢倾向。
Membrane fouling is the major drawback of membrane-based technologies because it will lead to severe flux declines and the need to clean or replace the fouled membrane. A technique capable of early diagnosis, process monitoring, and evaluation of the role of different foulants playing in the fouling process is crucial for the fouling control. We develop surface-enhanced Raman spectroscopy (SERS) as a new and versatile tool to investigate the fouling process of protein on PVDF (polyvinylidene fluoride) membranes as well as the fouling propensity of three different proteins. We optimized the aggregation level and volume of SERS-active Ag sol and the spectra acquisition method combined with a statistical analysis method to ensure a high detection sensitivity, signal uniformity, and stability. We then used SEAS for the early diagnosis of the fouling process and determining when the membrane pores would be blocked. The fouled area was visualized by a combination of the silver staining and Raman mapping. The fouling propensity of different proteins was studied by comparing the relative SERS band intensities of different proteins on a glass slide and after membrane filtration. Compared with fluorescence-based techniques, the narrow, well-resolved Raman band, especially the use of the same excitation line and laser power, endows SEAS the ability to compare the fouling propensity in a very simple way.