High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array.
High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array.
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DOI：
10.1364/boe.1.001408
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发表时间：
2010-11-15
影响因子：
3.4
通讯作者：
Michalet X
中科院分区：
文献类型：
--
作者：
Colyer RA;Scalia G;Rech I;Gulinatti A;Ghioni M;Cova S;Weiss S;Michalet X
我们提出了一种高通量荧光相关光谱(FCS)的新方法，使我们能够在采集时间上获得一个数量级的改进。该方法利用硅基液晶空间光调制器产生动态可调的焦斑，并采用8像素单光子雪崩光电二极管阵列。我们通过展示罗丹明6G在不同粘度下的FCS来展示该系统的能力，并通过展示通过对每个检测通道的适当校准，采集速度获得了一个数量级的提高。更广泛地说，我们的方法将允许进行更高吞吐量的单分子研究。
We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.