Simultaneous determination of urinary 31 metabolites of VOCs, 8-hydroxy-2'-deoxyguanosine, and trans-3'-hydroxycotinine by UPLC-MS/MS: 13C- and 15N-labeled isotoped internal standards are more effective on reduction of matrix effect.
Simultaneous determination of urinary 31 metabolites of VOCs, 8-hydroxy-2'-deoxyguanosine, and trans-3'-hydroxycotinine by UPLC-MS/MS: 13C- and 15N-labeled isotoped internal standards are more effective on reduction of matrix effect.
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DOI：
10.1007/s00216-019-02202-5
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发表时间：
2019
影响因子：
4.3
通讯作者：
Ruifang Fan
中科院分区：
文献类型：
--
作者：
Hongxuan Kuang;Yonghong Li;Wenhui Jiang;Peiqiong Wu;Jianhua Tan;Haibin Zhang;Qihua Pang;Shengtao Ma;Taicheng An;Ruifang Fan
人类不可避免地会接触到人为排放的挥发性有机化合物（VOC），因为它们是无处不在的大气污染物。吸烟是公众接触VOCs的重要途径。VOC暴露引起的健康效应引起了更多的关注，因为它们具有致癌性、遗传毒性、神经毒性和生殖毒性。反式-3 '-羟基可替宁（OH-Cot）是吸烟的尿生物标志物，8-羟基-2'-脱氧鸟苷（8-OHDG）是DNA氧化损伤的尿生物标志物。为建立一种可用于定量测定人群VOC暴露水平和评估二手烟VOCs健康风险的方法，建立了一种高效、快速、高通量的固相萃取-超高效液相色谱-串联质谱法（SPECT-MS/MS）同时测定VOCs代谢产物8-OHDG和OH-Cot的方法。大多数分析物的方法精密度和准确度、提取回收率、基质效应和储存稳定性均符合标准（80-120%）。经同位素内标调整后，萃取回收率从85.1%提高到100%。此外，13 C-和15 N-标记的IS比氘代类似物更有效地减少基质效应对回收率和精密度的影响（73.0-116% vs. 53.6-140%）。该方法已成功应用于儿童尿样的测定。结果表明，N-乙酰基-S-（3，4-二氢丁基）-L-半胱氨酸、2，2 ′-硫代二乙酸（TGA）和N-乙酰基-S-（3-羟丙基-1-甲基）-L-半胱氨酸（HPMMA）与8-OHDG的相关系数均大于0.82，表明这些挥发性有机化合物易导致DNA损伤。总之，我们在一种方法中与8-OHDG和OH-Cot共同监测VOC的代谢物提供了一种稳健的分析方法，该方法不仅表明了VOC引起的潜在不良健康影响，而且还区分和评估了被动吸烟在人体VOC暴露中的贡献。图形摘要。
Human beings are inevitably exposed to volatile organic compounds (VOCs) of anthropogenic emissions as they are ubiquitous atmospheric pollutants. Smoking is an important exposure route of VOCs for the general population. Health effects induced by VOC exposure raise more concerns as they are identified with carcinogenicity, genotoxicity, neurotoxicity, and reproductive toxicity. trans-3'-Hydroxycotinine (OH-Cot) is a urinary biomarker of smoking, and 8-hydroxy-2'-deoxyguanosine (8-OHDG) is a urinary biomarker of DNA oxidative damage. To develop a method for quantifying VOC exposure levels of the general population and assessing the health risks induced by VOCs from second-hand smoking, an effective, rapid, and high-throughput method for the simultaneous determination of 31 metabolites of VOCs, 8-OHDG, and OH-Cot using solid-phase extraction coupled with UPLC-MS/MS was developed and validated. Method precision and accuracy, extraction recoveries, matrix effects, and storage stabilities of most analytes met the criterion (80-120%). Extraction recoveries increased from 85.1 to 100% after adjustment by isotoped internal standards (ISs). Furthermore, 13C- and 15N-labeled ISs were more effective to reduce the influence of matrix effects on recoveries and precisions than the deuterated analogs (73.0-116% vs. 53.6-140%). This developed method was successfully applied to determine urine samples collected from children. Results showed that N-acetyl-S-(3,4-dihydrobutyl)-L-cysteine, 2,2'-thiodiacetic acid (TGA), and N-acetyl-S-(3-hydroxypropyl-1-methyl)-L-cysteine (HPMMA) were well correlated with 8-OHDG with coefficients higher than 0.82, indicating those VOCs might easily lead to DNA damage. In conclusion, our co-monitoring of metabolites of VOCs with 8-OHDG and OH-Cot in one method provides a robust analytical method, which not only suggests the potential adverse health effects induced by VOCs but also discriminates and evaluates the contribution of passive smoking in human VOC exposure. Graphical abstract.