Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation.
Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation.
复制标题
DOI：
10.1016/j.molcel.2017.02.018
复制
发表时间：
2017-03-16
期刊：
影响因子：
16
通讯作者：
Goldstein B
中科院分区：
文献类型：
--
作者：
Boothby TC;Tapia H;Brozena AH;Piszkiewicz S;Smith AE;Giovannini I;Rebecchi L;Pielak GJ;Koshland D;Goldstein B
迟滞是一种微小的动物，它们能在包括干燥在内的各种压力下存活下来。250多年来，迟滞是如何在干旱中幸存下来的一直是一个谜。海藻糖是一种对几种生物在干燥中存活所必需的二糖，在一些迟滞物种中检测到低水平或根本没有，这表明迟滞具有潜在的存活干燥的新机制。在这里，我们展示了迟滞特异的内在无序蛋白(TDP)对于脱水耐受性是必不可少的。在多种迟发性物种中，TDP基因在干燥过程中高水平结构性表达或诱导表达。TDP是迟滞脱水耐受性所必需的，这些基因在异源系统中表达时足以提高脱水耐受性。TDP在干燥时形成非晶态无定形固体(玻璃化)，这种玻璃化状态反映了它们的保护能力。我们的研究确定TDPs是迟滞干燥耐受性的功能介质，扩大了我们对参与应激耐受性的无序蛋白的作用和多样性的了解。迟滞(水熊)经受住了许多极端的压力，包括干燥。Boothby等人。证明迟滞无序蛋白是耐脱水所必需的，并从外源上保护细胞和纯化的酶不会干燥。干燥后，这些蛋白质形成玻璃，玻璃的完整性与它们的保护能力相关。
Tardigrades are microscopic animals that survive a remarkable array of stresses, including desiccation. How tardigrades survive desiccation has remained a mystery for more than 250 years. Trehalose, a disaccharide essential for several organisms to survive drying, is detected at low levels or not at all in some tardigrade species, indicating that tardigrades possess potentially novel mechanisms for surviving desiccation. Here we show that tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance. TDP genes are constitutively expressed at high levels or induced during desiccation in multiple tardigrade species. TDPs are required for tardigrade desiccation tolerance, and these genes are sufficient to increase desiccation tolerance when expressed in heterologous systems. TDPs form non-crystalline amorphous solids (vitrify) upon desiccation, and this vitrified state mirrors their protective capabilities. Our study identifies TDPs as functional mediators of tardigrade desiccation tolerance, expanding our knowledge of the roles and diversity of disordered proteins involved in stress tolerance. Tardigrades (water bears) survive a number of extreme stresses, including desiccation. Boothby et al. show that tardigrade disordered proteins are required for desiccation tolerance and exogenously protect cells and purified enzymes from drying. When dry, these proteins form glasses, the integrity of which correlates with their protective capabilities.
登录
查看更多内容
DOI：
10.1111/j.1439-0469.2010.00607.x
发表时间：
2011-05-01
影响因子：
1.9
作者：
Altiero, Tiziana;Guidetti, Roberto;Rebecchi, Lorena
通讯作者：
Rebecchi, Lorena
影响因子：
9.2
作者：
Erkut, Cihan;Penkov, Sider;Kurzchalia, Teymuras V.
通讯作者：
Kurzchalia, Teymuras V.
影响因子：
4.1
作者：
Goyal, K;Walton, LJ;Tunnacliffe, A
通讯作者：
Tunnacliffe, A
DOI：
10.1016/s1095-6433(01)00503-7
发表时间：
2002-03-01
影响因子：
2.3
作者：
Crowe, LM
通讯作者：
Crowe, LM
影响因子：
14.8
作者：
通讯作者：
--