Biocompatible, biodegradable and sterically stabilized phospholipid nanomicelles improve cryopreservation of oral keratinocytes: a preliminary investigation.
Biocompatible, biodegradable and sterically stabilized phospholipid nanomicelles improve cryopreservation of oral keratinocytes: a preliminary investigation.
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生物相容性、可生物降解和空间稳定的磷脂纳米胶束改善口腔角质形成细胞的冷冻保存：初步研究。
DOI：
10.1016/j.ijpharm.2007.02.002
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发表时间：
2007
影响因子：
5.8
通讯作者：
Onyuksel,Hayat
中科院分区：
文献类型：
--
作者：
Rubinstein,Israel;Onyuksel,Hayat
在这项原理验证研究中，我们确定了生物相容性、生物可降解性和空间稳定性的磷脂纳米胶束（SSNM）是否能改善冷冻保存的口腔角质形成细胞的活力和膜完整性。将培养的化学转化的仓鼠口腔角质形成细胞在10%二甲基亚砜（DMSO）或由二硬脂酰磷脂酰-乙醇胺-N-聚乙二醇2000（大小，17± 1 nm; 0.1和1.0nmol）组成的SSNM存在下逐渐冷冻并储存在液氮中。48小时后，解冻细胞并测定其活力。还定量细胞裂解物和上清液中的乳酸脱氢酶（LDH）活性。与10%DMS0相比，SSNM引起细胞活力的显著的浓度依赖性增加（p<0.05）。与10%DMSO相比，用SSNM冷冻保存的细胞上清液中的LDH活性也显著降低（p<0.05）。这些数据表明，SSNM通过促进细胞活力和质膜完整性来改善口腔角质形成细胞的冷冻保存。我们建议，SSNMs应进一步发展为一种新的角质形成细胞的纳米冷冻保存剂。
In this proof-of principle study, we determined whether biocompatible, biodegradable and sterically stabilized phospholipid nanomicelles (SSNMs) improve viability and membrane integrity of cryopreserved oral keratinocytes. Cultured chemically transformed hamster oral keratinocytes were frozen gradually with and stored in liquid nitrogen in the presence of 10% dimethylsulfoxide (DMSO) or SSNMs composed of distearoylphosphatidyl-ethanolamine-N-poly(ethylene glycol) 2000 (size, 17±1nm; 0.1 and 1.0nmol). Forty-eight hours later, cells were thawed and their viability was determined. Lactate dehydrogenase (LDH) activity in cell lysates and supernatants was quantified as well. SSNMs evoked a significant, concentration-dependant increase in cell viability in comparison to 10% DMSO (p<0.05). There was also a significant decrease in LDH activity in the supernatant of cells cryopreserved with SSNMs in comparison to 10% DMSO (p<0.05). These data indicate that SSNMs improve cryopreservation of oral keratinocyte by promoting cell viability and plasma membrane integrity. We suggest that SSNMs should be further developed as a novel nanocryopreservative for keratinocytes.